Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 4893875..4894391 Replicon chromosome
Accession NZ_CP034039
Organism Klebsiella pneumoniae subsp. pneumoniae strain CRK0298

Toxin (Protein)


Gene name relE Uniprot ID R4YAY3
Locus tag C5X33_RS24560 Protein ID WP_004178374.1
Coordinates 4893875..4894159 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID R4Y888
Locus tag C5X33_RS24565 Protein ID WP_002886901.1
Coordinates 4894149..4894391 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5X33_RS24540 4889286..4889600 - 315 WP_105446383.1 PTS sugar transporter subunit IIB -
C5X33_RS30280 4889685..4889858 + 174 WP_019725541.1 hypothetical protein -
C5X33_RS24545 4889861..4890604 + 744 WP_002886905.1 MurR/RpiR family transcriptional regulator -
C5X33_RS24550 4890961..4893099 + 2139 WP_019725540.1 anaerobic ribonucleoside-triphosphate reductase -
C5X33_RS24555 4893407..4893871 + 465 WP_004178375.1 anaerobic ribonucleoside-triphosphate reductase-activating protein -
C5X33_RS24560 4893875..4894159 - 285 WP_004178374.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C5X33_RS24565 4894149..4894391 - 243 WP_002886901.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
C5X33_RS24570 4894469..4896379 - 1911 WP_019725539.1 BglG family transcription antiterminator -
C5X33_RS24575 4896402..4897556 - 1155 WP_004178372.1 lactonase family protein -
C5X33_RS24580 4897623..4898363 - 741 WP_002886899.1 KDGP aldolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-80)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 11155.97 Da        Isoelectric Point: 10.3787

>T115037 WP_004178374.1 NZ_CP034039:c4894159-4893875 [Klebsiella pneumoniae subsp. pneumoniae]
MTYELEFDPRAWREWQKPGETVKKQFKNKLQQIVQNPRIESTRLSDLPDCYKIKLKASGYRLVYQVRDSVVVVYVIAIGK
REKAAVYHQANKRL

Download         Length: 285 bp

>T115037 NZ_CP034039:c4894159-4893875 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCTGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9045.55 Da        Isoelectric Point: 6.3340

>AT115037 WP_002886901.1 NZ_CP034039:c4894391-4894149 [Klebsiella pneumoniae subsp. pneumoniae]
MATLNVRLDDKLKKEAYAVLEKLDMTPTEAVRLLFQYIAENGRMPVKSMTISDGEAALLRTVRERLENPRPGVKVSLDDL

Download         Length: 243 bp

>AT115037 NZ_CP034039:c4894391-4894149 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A6THG1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GLP0

References