Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) ykfI-yfjZ/CbtA-CbeA
Location 4809670..4810361 Replicon chromosome
Accession NZ_CP034039
Organism Klebsiella pneumoniae subsp. pneumoniae strain CRK0298

Toxin (Protein)


Gene name ykfI Uniprot ID A0A483YFJ1
Locus tag C5X33_RS24165 Protein ID WP_025987721.1
Coordinates 4809670..4810011 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID A0A2A5MHA6
Locus tag C5X33_RS24170 Protein ID WP_019725272.1
Coordinates 4810035..4810361 (-) Length 109 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5X33_RS24150 4806643..4807878 - 1236 WP_124044824.1 hypothetical protein -
C5X33_RS24155 4808324..4808635 - 312 WP_073568733.1 hypothetical protein -
C5X33_RS24160 4808722..4809555 - 834 WP_025987720.1 DUF4942 domain-containing protein -
C5X33_RS24165 4809670..4810011 - 342 WP_025987721.1 TA system toxin CbtA family protein Toxin
C5X33_RS24170 4810035..4810361 - 327 WP_019725272.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
C5X33_RS24175 4810375..4810851 - 477 WP_019725273.1 DNA repair protein RadC -
C5X33_RS24180 4810861..4811307 - 447 WP_025987722.1 antirestriction protein -
C5X33_RS24185 4811519..4812208 - 690 WP_009309812.1 hypothetical protein -
C5X33_RS24195 4812773..4813663 - 891 WP_019725276.1 50S ribosome-binding GTPase -
C5X33_RS24200 4813746..4814834 - 1089 WP_019725277.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4792602..4835703 43101


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-107)

Antitoxin

Antitoxin CbeA

(8-107)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12867.90 Da        Isoelectric Point: 8.5195

>T115036 WP_025987721.1 NZ_CP034039:c4810011-4809670 [Klebsiella pneumoniae subsp. pneumoniae]
MKTLPATIQRAAKPCPSPVTVWQMLLTRLLEQHYGLTLNDTPFSDETVIQEHINAGITLVDAVNFLVEKYELVRIDRRGF
NCQEQSPYLGAVDILRARQATGLLRQRHLPSIR

Download         Length: 342 bp

>T115036 NZ_CP034039:c4810011-4809670 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAAACCTTACCTGCAACAATTCAGCGGGCGGCTAAGCCGTGCCCGTCTCCCGTGACGGTCTGGCAGATGCTGCTTAC
CCGTCTGCTGGAGCAACATTATGGCCTTACCCTGAATGACACGCCGTTCAGCGATGAAACCGTTATTCAGGAACATATCA
ACGCAGGCATCACACTGGTTGATGCCGTTAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCGCAGAGGGTTT
AACTGTCAGGAACAGTCACCTTATCTGGGCGCAGTGGATATTTTGCGGGCGCGGCAGGCAACCGGCCTGTTACGGCAAAG
ACATCTTCCTTCGATACGCTGA

Antitoxin


Download         Length: 109 a.a.        Molecular weight: 12276.01 Da        Isoelectric Point: 6.9560

>AT115036 WP_019725272.1 NZ_CP034039:c4810361-4810035 [Klebsiella pneumoniae subsp. pneumoniae]
MPLTDAIQWGLKRSFTPNFGARLVQEGNRLHYLADRANITGKFGDAECLKLEEAFPHFIRQMELMLSTGELNPRHAHSVT
LYHNAFTCEADTLGSCGYVYIVIYPTQR

Download         Length: 327 bp

>AT115036 NZ_CP034039:c4810361-4810035 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCACTGACTGATGCCATACAGTGGGGATTAAAGCGAAGTTTCACACCGAATTTTGGCGCAAGGCTGGTACAGGAAGG
CAACCGACTGCACTACCTGGCTGACCGGGCCAATATCACCGGTAAATTTGGTGACGCCGAATGTCTGAAGCTGGAAGAAG
CATTCCCGCATTTTATCCGCCAGATGGAACTGATGCTGAGCACTGGCGAACTTAATCCCCGTCATGCGCACAGCGTCACT
CTCTACCACAACGCTTTCACCTGTGAAGCCGATACTCTCGGTAGTTGTGGTTACGTCTACATCGTTATTTATCCCACTCA
ACGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483YFJ1

Antitoxin

Source ID Structure
AlphaFold DB A0A2A5MHA6

References