Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4146969..4147588 Replicon chromosome
Accession NZ_CP034039
Organism Klebsiella pneumoniae subsp. pneumoniae strain CRK0298

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag C5X33_RS20920 Protein ID WP_002892050.1
Coordinates 4147370..4147588 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag C5X33_RS20915 Protein ID WP_002892066.1
Coordinates 4146969..4147343 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5X33_RS20905 4142121..4143314 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
C5X33_RS20910 4143337..4146483 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
C5X33_RS20915 4146969..4147343 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
C5X33_RS20920 4147370..4147588 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
C5X33_RS20925 4147747..4148313 + 567 WP_002892030.1 maltose O-acetyltransferase -
C5X33_RS30140 4148285..4148425 - 141 WP_004147370.1 hypothetical protein -
C5X33_RS20930 4148446..4148916 + 471 WP_002892026.1 YlaC family protein -
C5X33_RS20935 4148891..4150342 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
C5X33_RS20940 4150443..4151141 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
C5X33_RS20945 4151138..4151278 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
C5X33_RS20950 4151278..4151541 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T115034 WP_002892050.1 NZ_CP034039:4147370-4147588 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T115034 NZ_CP034039:4147370-4147588 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT115034 WP_002892066.1 NZ_CP034039:4146969-4147343 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT115034 NZ_CP034039:4146969-4147343 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References