Detailed information of TA system TA115019

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 454521..455158
• Replicon: chromosome
• Accession: NZ_CP034037
• Organism: Bacillus velezensis strain BCSo1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: EG219_RS02325
• Protein ID: WP_003156187.1
• Coordinates: 454808..455158 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: EG219_RS02320
• Protein ID: WP_003156188.1
• Coordinates: 454521..454802 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EG219_RS02300	450886..451485	-	600	WP_020953896.1	rhomboid family intramembrane serine protease	-
EG219_RS02305	451578..451943	+	366	WP_007410229.1	holo-ACP synthase	-
EG219_RS02310	452108..453115	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
EG219_RS02315	453232..454401	+	1170	WP_007410231.1	alanine racemase	-
EG219_RS02320	454521..454802	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
EG219_RS02325	454808..455158	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
EG219_RS02330	455279..456100	+	822	WP_007410232.1	STAS domain-containing protein	-
EG219_RS02335	456105..456470	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
EG219_RS02340	456473..456874	+	402	WP_003156178.1	anti-sigma regulatory factor	-
EG219_RS02345	456886..457893	+	1008	WP_007410233.1	PP2C family protein-serine/threonine phosphatase	-
EG219_RS02350	457957..458286	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
EG219_RS02355	458283..458765	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
EG219_RS02360	458731..459519	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
EG219_RS02365	459519..460121	+	603	WP_007410234.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T115019 WP_003156187.1 NZ_CP034037:454808-455158 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T115019 NZ_CP034037:454808-455158 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT115019 WP_003156188.1 NZ_CP034037:454521-454802 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT115019 NZ_CP034037:454521-454802 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5