Detailed information of TA system TA115017

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 4636183..4636817
• Replicon: chromosome
• Accession: NZ_CP034036
• Organism: Brenneria nigrifluens DSM 30175 = ATCC 13028

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: EH206_RS21790
• Protein ID: WP_083832821.1
• Coordinates: 4636183..4636458 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: G7LSS7
• Locus tag: EH206_RS21795
• Protein ID: WP_009114924.1
• Coordinates: 4636455..4636817 (+)
• Length: 121 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EH206_RS21775	4631666..4632812	+	1147	Protein_4206	DNA cytosine methyltransferase	-
EH206_RS21780	4632812..4635325	+	2514	WP_009114921.1	replication endonuclease	-
EH206_RS21785	4635419..4636114	+	696	WP_009114922.1	DNA adenine methylase	-
EH206_RS21790	4636183..4636458	+	276	WP_083832821.1	type II toxin-antitoxin system HicA family toxin	Toxin
EH206_RS21795	4636455..4636817	+	363	WP_009114924.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EH206_RS21800	4637284..4638336	-	1053	WP_009114925.1	phage portal protein	-
EH206_RS21805	4638338..4640059	-	1722	WP_009114926.1	terminase family protein	-
EH206_RS21810	4640224..4641057	+	834	WP_009114927.1	GPO family capsid scaffolding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4625232..4658448	33216

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-85)

Antitoxin

• Uncharacterised protein family HicB (61-113)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10171.75 Da
• Isoelectric Point: 10.9754

>T115017 WP_083832821.1 NZ_CP034036:4636183-4636458 [Brenneria nigrifluens DSM 30175 = ATCC 13028]
MQEKIASLRKKQRETLNQIFKTPVLSGVKWSNIESLITALGGEIKEGSGSRVRFFLNGSIARFHRPHPSPDTDKGALVSL
REWLESIGVKP

Nucleotide

• Length: 276 bp

>T115017 NZ_CP034036:4636183-4636458 [Brenneria nigrifluens DSM 30175 = ATCC 13028]
ATGCAGGAAAAAATAGCCAGTCTGAGAAAAAAACAGCGGGAAACGCTGAATCAGATTTTCAAAACGCCGGTACTCTCTGG
TGTTAAATGGTCGAATATTGAGTCACTTATCACTGCGTTAGGTGGTGAGATTAAGGAAGGAAGCGGCTCAAGAGTACGAT
TTTTTCTGAACGGGAGCATTGCCCGATTCCATCGTCCGCATCCGTCACCGGATACTGATAAAGGCGCGTTGGTCAGCCTG
CGTGAATGGTTGGAAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 121 a.a.
• Molecular weight: 13377.26 Da
• Isoelectric Point: 4.4777

>AT115017 WP_009114924.1 NZ_CP034036:4636455-4636817 [Brenneria nigrifluens DSM 30175 = ATCC 13028]
MTKALNTPNTMVVAGQPAIISYVPEIGMFRGKFIGLSGYCDFVADSIDGLRSEGEISLREYLADCQESGIDPYEREEKVK
TFTLRYPESFGERLTIAAAERQVSVNAFIVETLNERMKLA

Nucleotide

• Length: 363 bp

>AT115017 NZ_CP034036:4636455-4636817 [Brenneria nigrifluens DSM 30175 = ATCC 13028]
ATGACTAAAGCACTTAATACACCAAATACGATGGTTGTTGCCGGGCAACCGGCCATCATTAGTTATGTGCCTGAAATTGG
CATGTTTCGTGGTAAGTTTATTGGTTTATCAGGCTATTGTGACTTTGTAGCCGATAGCATTGATGGGCTAAGGAGTGAGG
GAGAAATCTCACTACGTGAATATCTGGCAGATTGTCAGGAAAGCGGAATCGATCCCTATGAGCGTGAAGAGAAAGTGAAA
ACGTTCACCCTCCGCTACCCTGAATCGTTCGGAGAACGGCTGACCATTGCCGCCGCTGAACGCCAGGTTTCAGTGAATGC
GTTTATCGTCGAAACGCTTAACGAACGAATGAAGCTCGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A2U1UQB7