Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3941057..3941708 Replicon chromosome
Accession NZ_CP034036
Organism Brenneria nigrifluens DSM 30175 = ATCC 13028

Toxin (Protein)


Gene name cptA Uniprot ID A0A2U1URZ2
Locus tag EH206_RS18450 Protein ID WP_040343431.1
Coordinates 3941057..3941461 (-) Length 135 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID G7LQQ3
Locus tag EH206_RS18455 Protein ID WP_009114398.1
Coordinates 3941442..3941708 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EH206_RS18425 3936058..3936774 - 717 WP_009114392.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EH206_RS18430 3936851..3937750 - 900 WP_009114393.1 site-specific tyrosine recombinase XerD -
EH206_RS18435 3938068..3939393 + 1326 WP_009114394.1 MHS family MFS transporter -
EH206_RS18440 3939516..3940034 + 519 WP_009114395.1 flavodoxin FldB -
EH206_RS18445 3940041..3940991 - 951 WP_009114396.1 23S rRNA (adenine(1618)-N(6))-methyltransferase RlmF -
EH206_RS18450 3941057..3941461 - 405 WP_040343431.1 protein YgfX Toxin
EH206_RS18455 3941442..3941708 - 267 WP_009114398.1 FAD assembly factor SdhE Antitoxin
EH206_RS18460 3941925..3942911 + 987 WP_009114399.1 tRNA-modifying protein YgfZ -
EH206_RS18465 3943053..3943295 + 243 WP_009114400.1 DUF4160 domain-containing protein -
EH206_RS18470 3943279..3943527 + 249 WP_009114401.1 DUF2442 domain-containing protein -
EH206_RS18475 3943632..3944279 - 648 WP_009114402.1 hemolysin III family protein -
EH206_RS18480 3944591..3945199 + 609 WP_009114403.1 HD domain-containing protein -
EH206_RS18485 3945280..3946587 - 1308 WP_009114404.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(2-132)

Antitoxin

Flavinator of succinate dehydrogenase

(8-79)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 15865.72 Da        Isoelectric Point: 11.8263

>T115011 WP_040343431.1 NZ_CP034036:c3941461-3941057 [Brenneria nigrifluens DSM 30175 = ATCC 13028]
VAQWQCDLRVSWRMQLFSLVVHGLLVLLILLAPWPDSYTALWLGLVTLVVFGFIRSQRNIKVRQGEIALRGATHLRWQQR
DWQIIKRPWVLKSGILLSLKSMDGKERQRLWLASDSMGNDEWRHLRQLLLQQRN

Download         Length: 405 bp

>T115011 NZ_CP034036:c3941461-3941057 [Brenneria nigrifluens DSM 30175 = ATCC 13028]
GTGGCCCAGTGGCAATGTGACCTTCGCGTTTCCTGGCGTATGCAGCTGTTCTCACTGGTTGTACACGGATTACTGGTTTT
ACTGATACTGCTGGCTCCCTGGCCCGATAGCTATACCGCGCTTTGGCTGGGGCTGGTGACGCTGGTGGTGTTCGGTTTTA
TCCGCAGCCAGCGCAACATCAAGGTTCGCCAGGGGGAGATTGCTCTGCGCGGAGCAACGCACCTGCGCTGGCAGCAGCGG
GACTGGCAGATTATCAAACGTCCGTGGGTGCTGAAGAGCGGCATTCTGCTGTCGCTGAAATCGATGGATGGTAAAGAACG
GCAGCGTCTGTGGCTGGCGTCGGACAGCATGGGAAACGACGAGTGGCGCCATCTGCGCCAATTGCTGTTGCAGCAGAGAA
ATTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10464.98 Da        Isoelectric Point: 6.2171

>AT115011 WP_009114398.1 NZ_CP034036:c3941708-3941442 [Brenneria nigrifluens DSM 30175 = ATCC 13028]
MEIDNKSRIHWACRRGMRELDISIMPFFEHEYDALSDGDKRTFVRLLQCDDPDLFNWLMNHGEPQDQDLKRMISLIQTRN
KARGPVAM

Download         Length: 267 bp

>AT115011 NZ_CP034036:c3941708-3941442 [Brenneria nigrifluens DSM 30175 = ATCC 13028]
ATGGAAATCGATAATAAATCACGCATTCACTGGGCATGTCGGCGGGGTATGCGTGAGTTGGATATTTCTATCATGCCTTT
TTTTGAGCATGAGTATGATGCGCTAAGCGATGGCGACAAACGGACGTTCGTCCGCCTGCTGCAGTGTGACGATCCGGATC
TGTTTAACTGGCTGATGAATCACGGTGAACCGCAAGATCAGGATCTGAAACGTATGATCTCCCTTATCCAGACGCGAAAT
AAAGCCCGTGGCCCAGTGGCAATGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U1URZ2

Antitoxin

Source ID Structure
AlphaFold DB A0A2U1URW5

References