Detailed information of TA system TA114997

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 2626924..2627559
• Replicon: chromosome
• Accession: NZ_CP034036
• Organism: Brenneria nigrifluens DSM 30175 = ATCC 13028

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: G7LKZ1
• Locus tag: EH206_RS12375
• Protein ID: WP_009113102.1
• Coordinates: 2627383..2627559 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: G7LKZ0
• Locus tag: EH206_RS12370
• Protein ID: WP_009113101.1
• Coordinates: 2626924..2627331 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EH206_RS12335	2622459..2623481	-	1023	WP_009113093.1	terminase small subunit	-
EH206_RS12340	2623777..2624334	-	558	WP_009113095.1	DUF2514 domain-containing protein	-
EH206_RS12345	2624331..2624954	-	624	WP_009113096.1	glycoside hydrolase family 19 protein	-
EH206_RS12350	2624960..2625298	-	339	WP_198008350.1	phage holin, lambda family	-
EH206_RS12355	2625492..2625752	+	261	WP_009113098.1	hypothetical protein	-
EH206_RS12360	2625765..2625926	+	162	WP_009113099.1	hypothetical protein	-
EH206_RS12365	2625977..2626867	+	891	WP_009113100.1	tyrosine-type recombinase/integrase	-
EH206_RS12370	2626924..2627331	-	408	WP_009113101.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EH206_RS12375	2627383..2627559	-	177	WP_009113102.1	type II toxin-antitoxin system HicA family toxin	Toxin
EH206_RS12380	2627746..2628357	-	612	WP_009113103.1	DUF1133 family protein	-
EH206_RS12385	2628354..2628980	-	627	WP_009113104.1	recombination protein NinG	-
EH206_RS12390	2628973..2629422	-	450	WP_009113105.1	recombination protein NinB	-
EH206_RS12395	2629560..2629802	-	243	WP_009113106.1	hypothetical protein	-
EH206_RS12400	2629898..2631880	-	1983	WP_009113107.1	DNA cytosine methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-66)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6720.90 Da
• Isoelectric Point: 11.6500

>T114997 WP_009113102.1 NZ_CP034036:c2627559-2627383 [Brenneria nigrifluens DSM 30175 = ATCC 13028]
VKQSEFRRWLAAQGAEFRDGTNHLKIFLNGRQTIMPRHPGKEIPEPLRKAILKQLGLK

Nucleotide

• Length: 177 bp

>T114997 NZ_CP034036:c2627559-2627383 [Brenneria nigrifluens DSM 30175 = ATCC 13028]
GTGAAGCAAAGCGAGTTCAGGCGTTGGCTTGCAGCTCAAGGGGCAGAATTTAGGGATGGAACGAATCACCTTAAAATTTT
CCTCAACGGCAGGCAGACGATAATGCCGAGGCATCCGGGTAAAGAGATACCGGAGCCGCTTAGGAAAGCGATTCTTAAGC
AACTCGGATTGAAATAA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14639.63 Da
• Isoelectric Point: 4.2876

>AT114997 WP_009113101.1 NZ_CP034036:c2627331-2626924 [Brenneria nigrifluens DSM 30175 = ATCC 13028]
MQYPVTFDSDETGWAVLFPDIPEAMTGGDTREEALTMAQDALVTALDFYFEDRREIPTPSSTGEVFVEVPASVAAKVLLL
NAVVQQGVSNADLARLIGTRPQEVTRILDLHHATKIDTIQKALSALGKRLELAAA

Nucleotide

• Length: 408 bp

>AT114997 NZ_CP034036:c2627331-2626924 [Brenneria nigrifluens DSM 30175 = ATCC 13028]
ATGCAATATCCGGTAACGTTCGATAGCGATGAAACAGGGTGGGCGGTACTATTCCCCGATATCCCTGAAGCCATGACCGG
CGGCGACACCAGGGAGGAGGCGTTAACAATGGCGCAGGATGCTCTGGTTACAGCGCTGGACTTTTACTTTGAGGATCGGC
GTGAAATCCCAACACCGTCATCGACTGGCGAGGTGTTCGTGGAAGTGCCGGCCAGCGTAGCCGCTAAAGTCCTGCTGCTG
AACGCTGTGGTTCAGCAGGGTGTATCAAACGCTGACCTTGCGCGGTTGATCGGTACTCGGCCGCAGGAAGTAACGCGAAT
TCTCGATCTGCATCACGCGACGAAGATTGACACGATACAGAAAGCACTATCCGCGCTGGGTAAACGTCTGGAATTGGCCG
CCGCGTAA

Structures

Toxin

• AlphaFold DB: A0A2U1UU01

Antitoxin

• AlphaFold DB: A0A2U1UU21