Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2098726..2099364 Replicon chromosome
Accession NZ_CP034036
Organism Brenneria nigrifluens DSM 30175 = ATCC 13028

Toxin (Protein)


Gene name hicA Uniprot ID G7LX30
Locus tag EH206_RS09755 Protein ID WP_009112605.1
Coordinates 2098726..2098902 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A2U1UIL7
Locus tag EH206_RS09760 Protein ID WP_040343134.1
Coordinates 2098951..2099364 (+) Length 138 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EH206_RS09720 2093971..2095509 + 1539 WP_009112600.1 DEAD/DEAH box helicase -
EH206_RS09725 2095506..2096471 + 966 WP_009112601.1 toprim domain-containing protein -
EH206_RS09730 2096468..2096851 + 384 WP_083832775.1 DUF968 domain-containing protein -
EH206_RS09735 2096844..2097239 + 396 WP_009112602.1 antitermination protein -
EH206_RS09745 2097507..2098097 + 591 WP_009112603.1 prophage antirepressor -
EH206_RS09750 2098390..2098650 + 261 WP_009112604.1 hypothetical protein -
EH206_RS09755 2098726..2098902 + 177 WP_009112605.1 type II toxin-antitoxin system HicA family toxin Toxin
EH206_RS09760 2098951..2099364 + 414 WP_040343134.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
EH206_RS09765 2099540..2100367 + 828 WP_009112607.1 DNA/RNA non-specific endonuclease -
EH206_RS09770 2100465..2100773 + 309 WP_009112608.1 hypothetical protein -
EH206_RS09775 2100754..2101263 + 510 WP_040343136.1 lysozyme -
EH206_RS09780 2101275..2101796 + 522 WP_137739236.1 DUF2514 domain-containing protein -
EH206_RS09785 2101828..2102022 + 195 WP_009112611.1 hypothetical protein -
EH206_RS09790 2102095..2102349 + 255 WP_009112612.1 DNA polymerase III subunit theta -
EH206_RS09795 2102479..2103123 + 645 WP_009112613.1 hypothetical protein -
EH206_RS09800 2103266..2103880 + 615 WP_009112614.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2069841..2126731 56890


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-65)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6709.83 Da        Isoelectric Point: 11.4032

>T114995 WP_009112605.1 NZ_CP034036:2098726-2098902 [Brenneria nigrifluens DSM 30175 = ATCC 13028]
VKQSEFRRWLESQGVEVSNGTNHLKLRYNGKRSVMPRHPGAEIKEPLRKAILKQLGLK

Download         Length: 177 bp

>T114995 NZ_CP034036:2098726-2098902 [Brenneria nigrifluens DSM 30175 = ATCC 13028]
GTGAAGCAAAGCGAGTTCAGGCGGTGGCTTGAATCTCAGGGGGTTGAAGTTTCAAACGGTACGAATCATTTGAAGCTGAG
ATACAACGGGAAGCGAAGCGTAATGCCGAGACATCCCGGCGCTGAGATAAAAGAACCACTGCGAAAGGCCATACTTAAAC
AACTTGGCCTGAAATAA

Antitoxin


Download         Length: 138 a.a.        Molecular weight: 15047.18 Da        Isoelectric Point: 4.3233

>AT114995 WP_040343134.1 NZ_CP034036:2098951-2099364 [Brenneria nigrifluens DSM 30175 = ATCC 13028]
MRYPVNLELDPDSGGYVVSFLDIPEALTQGDTREEALKEAMDALVTAFEFYFEDNQRVPAPGNITGDYVEVPASIAAKVL
MLNAFVDSGLTQVELASRMGVKKQEVTRLFDLHHSTKIDTIQKAISAMGLRLELVAA

Download         Length: 414 bp

>AT114995 NZ_CP034036:2098951-2099364 [Brenneria nigrifluens DSM 30175 = ATCC 13028]
ATGCGATATCCAGTAAATTTAGAGTTAGATCCGGACTCAGGCGGCTATGTGGTTTCGTTCCTGGATATACCGGAAGCGCT
AACTCAGGGCGATACGCGAGAAGAGGCGTTAAAAGAGGCGATGGATGCGCTGGTAACAGCCTTCGAGTTTTATTTCGAGG
ATAACCAGCGTGTTCCTGCGCCCGGTAATATTACCGGTGATTACGTCGAAGTTCCGGCAAGCATCGCCGCTAAGGTTTTA
ATGCTGAATGCGTTTGTCGATTCTGGCTTAACTCAGGTTGAGCTGGCTTCGCGCATGGGTGTAAAAAAACAGGAGGTAAC
CCGACTGTTTGACCTACACCACTCGACGAAAATAGACACCATTCAAAAGGCGATCTCGGCTATGGGGCTGCGCTTGGAAT
TGGTCGCCGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U1UIS4

Antitoxin

Source ID Structure
AlphaFold DB A0A2U1UIL7

References