Detailed information of TA system TA114984

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1023763..1024391
• Replicon: chromosome
• Accession: NZ_CP034035
• Organism: Brenneria rubrifaciens strain 6D370

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: E0SD87
• Locus tag: EH207_RS04815
• Protein ID: WP_009114000.1
• Coordinates: 1023763..1023966 (-)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: EH207_RS04820
• Protein ID: WP_137712964.1
• Coordinates: 1024023..1024391 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EH207_RS04785	1019153..1019491	+	339	WP_002208627.1	P-II family nitrogen regulator	-
EH207_RS04790	1019528..1020814	+	1287	WP_175413640.1	ammonium transporter AmtB	-
EH207_RS04795	1020992..1021855	-	864	WP_137712961.1	acyl-CoA thioesterase II	-
EH207_RS04800	1022184..1022696	+	513	WP_137712962.1	YbaY family lipoprotein	-
EH207_RS04805	1022801..1023121	-	321	WP_137712963.1	MGMT family protein	-
EH207_RS04815	1023763..1023966	-	204	WP_009114000.1	hemolysin expression modulator Hha	Toxin
EH207_RS04820	1024023..1024391	-	369	WP_137712964.1	Hha toxicity modulator TomB	Antitoxin
EH207_RS04825	1025261..1028407	-	3147	WP_137712965.1	efflux RND transporter permease subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8133.51 Da
• Isoelectric Point: 8.8500

>T114984 WP_009114000.1 NZ_CP034035:c1023966-1023763 [Brenneria rubrifaciens]
MKKIDYLMRLRKCTTIDTLERVIEKNKYELSNDELEMFYSAADHRLAELTMNKLYDKVPTAVWKYVR

Nucleotide

• Length: 204 bp

>T114984 NZ_CP034035:c1023966-1023763 [Brenneria rubrifaciens]
ATGAAAAAAATCGACTATTTGATGCGTTTACGTAAATGCACAACCATTGACACGCTTGAACGCGTTATTGAGAAAAACAA
GTATGAACTCTCCAATGATGAGTTGGAGATGTTCTATTCCGCAGCGGACCACCGTCTGGCAGAACTGACGATGAACAAGC
TTTATGACAAAGTTCCCACCGCTGTATGGAAATACGTCCGTTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14045.76 Da
• Isoelectric Point: 4.2149

>AT114984 WP_137712964.1 NZ_CP034035:c1024391-1024023 [Brenneria rubrifaciens]
MDEYTPKHYDIAQLRFLCENLCGESIATLGDSSHGWVNDPTSAINIQLNELIEHIATFILTFKIKYPDENELSQQVEKYL
DDTYVLFSNYGINDAELRLWQKSKAELFGMFSEENICTPAKT

Nucleotide

• Length: 369 bp

>AT114984 NZ_CP034035:c1024391-1024023 [Brenneria rubrifaciens]
ATGGATGAGTACACACCGAAACATTATGATATTGCCCAACTCAGATTCTTATGTGAAAATCTGTGCGGCGAAAGCATTGC
AACGTTAGGTGATAGCAGTCACGGTTGGGTCAATGACCCTACATCTGCGATCAATATCCAATTAAATGAACTTATTGAGC
ATATCGCTACATTTATTCTCACATTTAAAATAAAATACCCTGACGAAAACGAGCTTTCCCAGCAGGTTGAAAAGTATTTA
GACGACACCTACGTTTTGTTTAGTAACTACGGAATCAATGACGCTGAACTACGTCTTTGGCAGAAATCTAAAGCTGAATT
ATTCGGAATGTTCTCAGAAGAGAACATCTGTACGCCTGCTAAAACTTAA

Structures

Toxin

• AlphaFold DB: A0A1X3RNQ0