Detailed information of TA system TA114982

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 434076..434666
• Replicon: chromosome
• Accession: NZ_CP034035
• Organism: Brenneria rubrifaciens strain 6D370

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: EH207_RS02035
• Protein ID: WP_137712517.1
• Coordinates: 434334..434666 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: EH207_RS02030
• Protein ID: WP_121588677.1
• Coordinates: 434076..434333 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EH207_RS02005	431463..431768	-	306	WP_121574721.1	type II toxin-antitoxin system toxin CcdB	-
EH207_RS02010	431771..431989	-	219	WP_121574722.1	type II toxin-antitoxin system antitoxin CcdA	-
EH207_RS02015	432230..432511	-	282	WP_137712514.1	helix-turn-helix domain-containing protein	-
EH207_RS02020	432660..432974	+	315	WP_137712515.1	helix-turn-helix domain-containing protein	-
EH207_RS02025	433161..433475	+	315	WP_137712516.1	helix-turn-helix domain-containing protein	-
EH207_RS02030	434076..434333	+	258	WP_121588677.1	antitoxin	Antitoxin
EH207_RS02035	434334..434666	+	333	WP_137712517.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EH207_RS02045	435475..436356	+	882	WP_137712518.1	integrase domain-containing protein	-
EH207_RS02050	436825..438096	-	1272	WP_137712519.1	integrase arm-type DNA-binding domain-containing protein	-
EH207_RS02060	438450..439025	-	576	WP_137712520.1	transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	htpB	414739..459030	44291

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (4-85)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11734.53 Da
• Isoelectric Point: 10.9269

>T114982 WP_137712517.1 NZ_CP034035:434334-434666 [Brenneria rubrifaciens]
MDRGEIWLVSLDPTAGHEQSGKRPVLIVSPAPFNKITRLPVVVPVTSGGNFARTAGFAVSLDGAGTKTTGVVRCDQPRTI
DMAARNGRRLERIPDSIVNEVLARLGTILS

Nucleotide

• Length: 333 bp

>T114982 NZ_CP034035:434334-434666 [Brenneria rubrifaciens]
ATGGACAGAGGGGAAATCTGGCTCGTGTCGCTTGATCCCACTGCCGGGCATGAGCAAAGCGGAAAACGCCCGGTGCTTAT
CGTTTCACCGGCACCGTTTAACAAGATCACCCGGTTGCCTGTGGTTGTTCCTGTCACCAGTGGTGGAAACTTTGCCCGAA
CGGCTGGTTTTGCCGTTTCACTGGATGGCGCGGGAACGAAAACGACAGGTGTTGTTCGCTGTGACCAGCCCAGAACCATT
GATATGGCAGCCAGAAACGGCAGAAGGCTGGAGCGTATCCCGGACAGTATCGTAAACGAGGTACTGGCCCGACTGGGGAC
AATCCTTAGCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9271.64 Da
• Isoelectric Point: 4.4341

>AT114982 WP_121588677.1 NZ_CP034035:434076-434333 [Brenneria rubrifaciens]
MYTTRLKKVGGSVMLAVPPAVLKTLDLSPDSEVGVTVDNGCLIIEPQKRPHYSLAELLAQCDPHAEISDEEREWIDAPAT
GKEIL

Nucleotide

• Length: 258 bp

>AT114982 NZ_CP034035:434076-434333 [Brenneria rubrifaciens]
ATGTATACCACTCGTCTGAAAAAAGTCGGAGGATCTGTCATGCTGGCAGTGCCTCCCGCCGTGCTGAAAACGCTGGATTT
GTCGCCGGATAGTGAGGTCGGTGTGACCGTTGATAATGGCTGCCTGATTATTGAGCCTCAAAAACGGCCTCACTATTCGC
TTGCCGAACTATTGGCGCAGTGCGATCCACATGCCGAAATAAGTGATGAAGAGCGGGAATGGATTGACGCGCCTGCCACA
GGGAAGGAGATCCTGTAA