Detailed information of TA system TA114980

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 423032..423816
• Replicon: chromosome
• Accession: NZ_CP034035
• Organism: Brenneria rubrifaciens strain 6D370

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: EH207_RS01965
• Protein ID: WP_137712507.1
• Coordinates: 423322..423816 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: EH207_RS01960
• Protein ID: WP_136155584.1
• Coordinates: 423032..423325 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EH207_RS01935	418233..418670	+	438	WP_137712504.1	DUF29 domain-containing protein	-
EH207_RS01945	419893..421013	+	1121	WP_137712505.1	IS3 family transposase	-
EH207_RS01950	421085..421273	+	189	WP_009114544.1	hypothetical protein	-
EH207_RS01955	421354..422613	-	1260	WP_137712506.1	tyrosine-type recombinase/integrase	-
EH207_RS01960	423032..423325	+	294	WP_136155584.1	DUF1778 domain-containing protein	Antitoxin
EH207_RS01965	423322..423816	+	495	WP_137712507.1	GNAT family N-acetyltransferase	Toxin
EH207_RS18060	423915..424073	+	159	WP_175413619.1	hypothetical protein	-
EH207_RS01975	424393..425400	+	1008	WP_137712508.1	nucleoid-associated protein	-
EH207_RS01980	425393..426319	+	927	WP_137712509.1	hypothetical protein	-
EH207_RS01985	426316..427452	+	1137	WP_137712510.1	hypothetical protein	-
EH207_RS01990	427724..428074	+	351	WP_137712511.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	htpB	414739..459030	44291

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (76-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (4-95)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17758.72 Da
• Isoelectric Point: 8.2830

>T114980 WP_137712507.1 NZ_CP034035:423322-423816 [Brenneria rubrifaciens]
MISAPEPLRAEHVLSLFCCGVESMDNWLKQRAMKNQVTGASRTFVSCDGSKVLAYYSLASSAVATNAVPGRFRRNMPDPI
PVVVLGRLAVDKSLHGQSVGRALVRDAGLRVIQVADTIGIRGMLVHTLSDEAREFYLRVGFEPSPIDPMMLMVTLGDLVG
SLSI

Nucleotide

• Length: 495 bp

>T114980 NZ_CP034035:423322-423816 [Brenneria rubrifaciens]
ATGATTTCCGCCCCTGAGCCACTCCGCGCCGAACATGTACTATCTTTATTCTGTTGCGGCGTGGAGTCTATGGACAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTCACTGGCGCATCACGAACTTTTGTCAGTTGCGATGGCTCAAAAGTGCTGG
CGTATTACTCATTGGCCTCCAGCGCTGTAGCAACGAATGCCGTCCCCGGACGTTTTCGTCGCAATATGCCCGATCCGATC
CCGGTAGTAGTGCTGGGACGTCTGGCAGTAGATAAATCATTGCATGGTCAGAGCGTTGGTCGGGCGCTAGTAAGGGATGC
GGGGCTGCGGGTGATTCAGGTGGCGGATACTATTGGCATTCGTGGGATGCTGGTTCATACGCTGTCTGATGAAGCGCGGG
AATTTTACCTGCGTGTGGGATTTGAGCCTTCACCGATTGACCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTGGGG
AGTTTATCAATTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10911.52 Da
• Isoelectric Point: 10.1968

>AT114980 WP_136155584.1 NZ_CP034035:423032-423325 [Brenneria rubrifaciens]
MPVTNNVSVKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIDQRIIMADPDAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT114980 NZ_CP034035:423032-423325 [Brenneria rubrifaciens]
ATGCCAGTGACGAACAACGTTTCTGTTAAACGCGAAACATTGAATTTACGCATCAAGCCCGCTGAACGCGATCTGATCGA
TCGTGCAGCGAAGGCCAGAGGGAAAAATCGTACTGATTTTGTCCTTGAGGCCGCTCGTGCTGCCGCAGAGGAAGCGTTGA
TCGATCAGCGCATCATCATGGCAGATCCCGATGCTTATCAGGAATTCCTTGCTCGTTTGGATCAGGCTCCTGCACCAAAT
GCCGCGCTGCGAAAAACCATGCAAACGCCTGCACCGTGGGAACAGAAAAAATGA