Detailed information of TA system TA114950

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2140984..2141513
• Replicon: chromosome
• Accession: NZ_CP034014
• Organism: Staphylococcus aureus strain P1D1C1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: EFD67_RS10630
• Protein ID: WP_000621175.1
• Coordinates: 2140984..2141346 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: EFD67_RS10635
• Protein ID: WP_000948331.1
• Coordinates: 2141343..2141513 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EFD67_RS10610	2137963..2138733	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
EFD67_RS10615	2138708..2139187	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
EFD67_RS10620	2139189..2139515	-	327	WP_001052491.1	anti-sigma factor antagonist	-
EFD67_RS10625	2139634..2140635	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
EFD67_RS10630	2140984..2141346	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EFD67_RS10635	2141343..2141513	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
EFD67_RS10640	2141598..2142746	-	1149	WP_001281154.1	alanine racemase	-
EFD67_RS10645	2142812..2143171	-	360	WP_000581197.1	holo-ACP synthase	-
EFD67_RS10650	2143175..2143666	-	492	WP_001205912.1	PH domain-containing protein	-
EFD67_RS10655	2143653..2145236	-	1584	WP_001294626.1	PH domain-containing protein	-
EFD67_RS10660	2145229..2145708	-	480	WP_001287087.1	hypothetical protein	-
EFD67_RS10665	2145916..2146476	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T114950 WP_000621175.1 NZ_CP034014:c2141346-2140984 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T114950 NZ_CP034014:c2141346-2140984 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT114950 WP_000948331.1 NZ_CP034014:c2141513-2141343 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT114950 NZ_CP034014:c2141513-2141343 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1