Detailed information of TA system TA114757

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 1245215..1245753
• Replicon: chromosome
• Accession: NZ_CP033972
• Organism: Gordonia insulae strain MMS17-SY073

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: D7316_RS05675
• Protein ID: WP_124707413.1
• Coordinates: 1245215..1245547 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: D7316_RS05680
• Protein ID: WP_124707414.1
• Coordinates: 1245544..1245753 (-)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D7316_RS05655	1241586..1242224	-	639	WP_124707409.1	hypothetical protein	-
D7316_RS05660	1242598..1243194	-	597	WP_124707410.1	hypothetical protein	-
D7316_RS05665	1243919..1244305	-	387	WP_124707411.1	hypothetical protein	-
D7316_RS05670	1244283..1244945	-	663	WP_124707412.1	nucleotidyltransferase domain-containing protein	-
D7316_RS05675	1245215..1245547	-	333	WP_124707413.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
D7316_RS05680	1245544..1245753	-	210	WP_124707414.1	DUF3018 family protein	Antitoxin
D7316_RS05685	1246001..1246930	+	930	WP_164473735.1	hypothetical protein	-
D7316_RS05690	1246993..1247409	+	417	WP_124707415.1	hypothetical protein	-
D7316_RS05695	1247455..1248123	-	669	WP_124707416.1	hypothetical protein	-
D7316_RS05700	1248271..1249542	-	1272	WP_124707417.1	FAD-dependent monooxygenase	-
D7316_RS27085	1249725..1249886	-	162	WP_164473736.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-105)

Antitoxin

• Antitoxin MazE-like (4-66)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11954.81 Da
• Isoelectric Point: 6.3330

>T114757 WP_124707413.1 NZ_CP033972:c1245547-1245215 [Gordonia insulae]
VIRGEIWTVAGGAYASKPRPAVIVQDDLFDSTLSVVVAPMTSQLVDAPLMRIRIRGDGDATSGLERDSDVMVDKLTAVRR
SNVLTRVGRLTSEQLVEVERSLMAFLGLAR

Nucleotide

• Length: 333 bp

>T114757 NZ_CP033972:c1245547-1245215 [Gordonia insulae]
GTGATTCGCGGTGAGATCTGGACAGTGGCGGGTGGTGCGTATGCGTCCAAGCCGCGGCCTGCGGTCATCGTCCAGGATGA
CCTTTTCGATTCAACTCTGTCGGTGGTGGTTGCGCCGATGACGAGCCAGCTTGTCGATGCGCCGTTGATGCGGATACGCA
TCCGAGGCGACGGGGACGCGACCTCGGGTCTCGAGCGGGACAGTGACGTGATGGTTGACAAGCTGACCGCGGTCAGACGG
TCGAACGTGCTGACGCGTGTTGGCCGGCTGACGTCCGAACAGCTGGTGGAAGTTGAACGCTCGCTGATGGCGTTCCTGGG
GCTCGCGCGATGA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 7939.86 Da
• Isoelectric Point: 4.4079

>AT114757 WP_124707414.1 NZ_CP033972:c1245753-1245544 [Gordonia insulae]
MAVRDRVGEYRRRMRERGLRPLQVWVPDVRTPEFAAQAHMQSLLVAQADGDGDEQDFVEAVAVPWDDET

Nucleotide

• Length: 210 bp

>AT114757 NZ_CP033972:c1245753-1245544 [Gordonia insulae]
ATGGCTGTGAGGGACAGGGTGGGTGAGTACCGACGCCGGATGCGTGAGCGCGGTTTGCGCCCGTTGCAGGTTTGGGTCCC
TGACGTGCGCACGCCCGAGTTTGCCGCGCAAGCCCACATGCAGTCGCTTCTCGTCGCGCAGGCCGATGGCGATGGGGACG
AGCAGGATTTCGTCGAGGCCGTTGCGGTGCCATGGGATGACGAGACGTGA