Detailed information of TA system TA114752

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3346238..3346894
• Replicon: chromosome
• Accession: NZ_CP033969
• Organism: Cupriavidus pauculus strain FDAARGOS_614

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: EHF44_RS16820
• Protein ID: WP_124684697.1
• Coordinates: 3346238..3346411 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: EHF44_RS16825
• Protein ID: WP_124684698.1
• Coordinates: 3346478..3346894 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EHF44_RS16790	3341823..3343211	-	1389	WP_124685155.1	DUF4055 domain-containing protein	-
EHF44_RS16795	3343269..3344570	-	1302	WP_124685156.1	terminase family protein	-
EHF44_RS16800	3344563..3345036	-	474	WP_124684694.1	DUF2280 domain-containing protein	-
EHF44_RS16805	3345067..3345720	-	654	WP_124684695.1	hypothetical protein	-
EHF44_RS16810	3345724..3345909	-	186	WP_124684696.1	hypothetical protein	-
EHF44_RS16820	3346238..3346411	+	174	WP_124684697.1	type II toxin-antitoxin system HicA family toxin	Toxin
EHF44_RS16825	3346478..3346894	+	417	WP_124684698.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EHF44_RS16835	3347192..3347476	-	285	WP_124684699.1	hypothetical protein	-
EHF44_RS16840	3348524..3348718	-	195	WP_124684700.1	hypothetical protein	-
EHF44_RS16845	3348726..3349160	-	435	WP_124684701.1	hypothetical protein	-
EHF44_RS16850	3349268..3349651	+	384	WP_124684702.1	hypothetical protein	-
EHF44_RS16855	3349834..3350709	+	876	WP_124684703.1	DUF1376 domain-containing protein	-
EHF44_RS16860	3350706..3351383	+	678	WP_124684704.1	hypothetical protein	-
EHF44_RS16865	3351425..3351622	+	198	WP_124684705.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3317847..3377595	59748

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-56)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6327.43 Da
• Isoelectric Point: 11.1504

>T114752 WP_124684697.1 NZ_CP033969:3346238-3346411 [Cupriavidus pauculus]
VKQSEFVRWLADQGATFKQGKKHLKVSLNGKQTVVPRHAAELKTGTVEAIKKQLGLK

Nucleotide

• Length: 174 bp

>T114752 NZ_CP033969:3346238-3346411 [Cupriavidus pauculus]
GTGAAGCAAAGTGAGTTTGTCCGTTGGCTAGCCGATCAAGGCGCTACCTTCAAGCAGGGCAAGAAACACCTGAAGGTTTC
CCTGAACGGCAAGCAAACGGTGGTGCCAAGACATGCTGCGGAGTTGAAGACCGGCACAGTCGAGGCGATCAAGAAACAAC
TCGGACTTAAGTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 14982.22 Da
• Isoelectric Point: 4.4194

>AT114752 WP_124684698.1 NZ_CP033969:3346478-3346894 [Cupriavidus pauculus]
MQYPALLEPASEGGFVVTFRDIPEAITQGDTEAEALDMATDALLTAMDFYFDDQRAVPAPSKARKGEQLVALPASVAAKV
LLLNEMVAQQIRPIDLAERLGTTKQEVNRLINLEHTTKIDRIGEALAVLGKHLDVVVA

Nucleotide

• Length: 417 bp

>AT114752 NZ_CP033969:3346478-3346894 [Cupriavidus pauculus]
ATGCAATATCCGGCACTGTTGGAACCTGCATCCGAAGGCGGCTTCGTCGTGACCTTCCGCGATATCCCCGAGGCGATCAC
TCAAGGGGATACCGAGGCGGAAGCCCTCGATATGGCTACCGACGCGCTTCTAACTGCGATGGACTTCTACTTTGACGACC
AGCGCGCGGTTCCGGCACCCTCGAAGGCTCGCAAAGGCGAGCAGTTGGTGGCGCTTCCTGCCAGCGTGGCGGCAAAGGTG
CTTCTACTGAACGAAATGGTCGCGCAGCAGATCCGCCCTATCGACCTGGCTGAACGACTGGGGACGACGAAGCAGGAAGT
GAATCGTCTCATCAACCTGGAGCACACCACAAAGATCGATCGGATTGGCGAGGCGCTGGCTGTTCTGGGCAAGCACCTGG
ACGTTGTCGTCGCGTAA