Detailed information of TA system TA114751

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1906311..1906959
• Replicon: chromosome
• Accession: NZ_CP033969
• Organism: Cupriavidus pauculus strain FDAARGOS_614

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: EHF44_RS10485
• Protein ID: WP_124683689.1
• Coordinates: 1906789..1906959 (-)
• Length: 57 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: EHF44_RS10480
• Protein ID: WP_124683688.1
• Coordinates: 1906311..1906727 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EHF44_RS10450	1901404..1902084	-	681	WP_172966036.1	hypothetical protein	-
EHF44_RS10455	1902192..1903364	-	1173	WP_124683684.1	hypothetical protein	-
EHF44_RS10460	1903395..1904189	-	795	WP_124683685.1	PD-(D/E)XK nuclease family protein	-
EHF44_RS28305	1904558..1904719	-	162	WP_172966037.1	hypothetical protein	-
EHF44_RS10465	1904746..1905093	+	348	WP_124683686.1	hypothetical protein	-
EHF44_RS10470	1905090..1905560	+	471	WP_124683687.1	RusA family crossover junction endodeoxyribonuclease	-
EHF44_RS10475	1905589..1906194	+	606	WP_172966104.1	DNA-binding protein	-
EHF44_RS10480	1906311..1906727	-	417	WP_124683688.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EHF44_RS10485	1906789..1906959	-	171	WP_124683689.1	type II toxin-antitoxin system HicA family toxin	Toxin
EHF44_RS10490	1907158..1908540	+	1383	WP_124683690.1	ParB N-terminal domain-containing protein	-
EHF44_RS10495	1908546..1908956	+	411	WP_124683691.1	hypothetical protein	-
EHF44_RS10500	1908907..1910457	+	1551	WP_124683692.1	phage terminase large subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1895003..1944983	49980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-55)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 57 a.a.
• Molecular weight: 6377.49 Da
• Isoelectric Point: 11.0962

>T114751 WP_124683689.1 NZ_CP033969:c1906959-1906789 [Cupriavidus pauculus]
VKRIEFVQWLAERGATFKEGSKHTKVYLNGKQTTIPRHTEIKIGTVNGIKKQLGLK

Nucleotide

• Length: 171 bp

>T114751 NZ_CP033969:c1906959-1906789 [Cupriavidus pauculus]
GTGAAGCGAATCGAGTTTGTCCAATGGCTGGCTGAACGGGGTGCGACCTTCAAAGAAGGCTCCAAACACACCAAGGTCTA
CCTGAACGGCAAGCAGACTACGATCCCCCGGCATACCGAGATCAAGATCGGTACGGTCAACGGAATCAAGAAACAACTAG
GACTCAAGTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 14979.19 Da
• Isoelectric Point: 4.5359

>AT114751 WP_124683688.1 NZ_CP033969:c1906727-1906311 [Cupriavidus pauculus]
MQYPATLTPDEGGGFVVTFRDIPEAITQGNTEAEALEMAADALLTSMDFYFEDKRPVPPPSKAKKGEQLVALPASAAAKV
LLLNEMLAQQVRPTDLAARMDTTKQEVNRLLDLGHTTKIDRVEEALAALGKRLELSLA

Nucleotide

• Length: 417 bp

>AT114751 NZ_CP033969:c1906727-1906311 [Cupriavidus pauculus]
ATGCAATATCCAGCAACCCTGACCCCCGACGAGGGTGGTGGCTTTGTCGTGACGTTCCGGGATATTCCCGAAGCGATCAC
GCAAGGCAATACCGAAGCCGAAGCACTCGAGATGGCCGCCGACGCGCTGTTGACGTCGATGGACTTCTACTTCGAAGACA
AGCGCCCTGTTCCCCCGCCGTCAAAGGCGAAGAAGGGCGAGCAGCTTGTCGCCTTGCCGGCCAGCGCGGCGGCGAAGGTG
CTGTTGCTCAACGAGATGCTGGCCCAGCAGGTACGACCGACCGATCTGGCGGCGCGCATGGACACGACCAAGCAGGAAGT
GAACCGCCTGCTGGACCTGGGCCATACAACGAAAATCGACCGTGTCGAAGAGGCGCTGGCCGCCTTGGGCAAACGCCTGG
AACTGAGCCTGGCATGA