Detailed information of TA system TA114750

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 141193..141758
• Replicon: plasmid unnamed1
• Accession: NZ_CP033968
• Organism: Cupriavidus pauculus strain FDAARGOS_614

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q58AR3
• Locus tag: EHF44_RS00810
• Protein ID: WP_011229306.1
• Coordinates: 141387..141758 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q58AR4
• Locus tag: EHF44_RS00805
• Protein ID: WP_011229305.1
• Coordinates: 141193..141390 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EHF44_RS00760	136436..136657	+	222	WP_011229302.1	hypothetical protein	-
EHF44_RS00765	136670..136912	+	243	WP_035882428.1	hypothetical protein	-
EHF44_RS00770	136909..137133	+	225	WP_011514790.1	hypothetical protein	-
EHF44_RS00775	137209..137538	+	330	WP_035882430.1	hypothetical protein	-
EHF44_RS00780	137570..138016	-	447	WP_011514792.1	hypothetical protein	-
EHF44_RS00785	138455..138763	-	309	WP_017514069.1	hypothetical protein	-
EHF44_RS00790	139052..139321	-	270	WP_124682103.1	hypothetical protein	-
EHF44_RS00795	139355..140305	-	951	WP_011229304.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
EHF44_RS00800	140302..141027	-	726	WP_011514795.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
EHF44_RS00805	141193..141390	+	198	WP_011229305.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
EHF44_RS00810	141387..141758	+	372	WP_011229306.1	type II toxin-antitoxin system VapC family toxin	Toxin
EHF44_RS00815	142148..142366	+	219	Protein_160	hypothetical protein	-
EHF44_RS00820	142504..143154	+	651	Protein_161	hypothetical protein	-
EHF44_RS00825	144199..144531	+	333	WP_111733968.1	hypothetical protein	-
EHF44_RS00830	144561..145064	+	504	WP_111733969.1	hypothetical protein	-
EHF44_RS00835	145129..145677	+	549	WP_038316447.1	hypothetical protein	-
EHF44_RS00840	145689..146237	-	549	WP_017513745.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cheY	1..312343	312343

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-113)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-44)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13613.83 Da
• Isoelectric Point: 5.6319

>T114750 WP_011229306.1 NZ_CP033968:141387-141758 [Cupriavidus pauculus]
MILVDTSVWIDHLNASDPMLISLLTQERVLIHPYVIGEISLGSLRDRDVVLGALRDLPRAPVATPEETLYLIEREGLFNR
GIGYGDTSLLASARLQPGIAIWTRDKRLKKVADELCLGAVLSH

Nucleotide

• Length: 372 bp

>T114750 NZ_CP033968:141387-141758 [Cupriavidus pauculus]
ATGATTCTTGTCGACACGTCGGTCTGGATTGACCACCTCAACGCTTCTGATCCGATGCTGATCAGCCTCTTAACCCAGGA
GCGTGTGCTGATTCATCCCTATGTGATCGGTGAGATTTCGCTTGGTAGTCTGCGGGACCGAGATGTCGTGCTTGGCGCTC
TGCGCGATTTGCCGCGAGCGCCTGTCGCAACACCAGAGGAGACCCTGTATTTGATCGAGCGCGAAGGCTTGTTCAACCGT
GGAATCGGATATGGCGATACATCATTGTTGGCATCCGCGCGTCTCCAACCAGGCATCGCCATCTGGACGCGTGACAAGCG
ATTGAAGAAAGTTGCTGATGAACTCTGTCTCGGCGCGGTCCTTTCGCACTAG

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7132.21 Da
• Isoelectric Point: 10.3509

>AT114750 WP_011229305.1 NZ_CP033968:141193-141390 [Cupriavidus pauculus]
MRTTIALDDELLAKAQAYTGLEEKTALVREALKALIQREAAKRLANLGGSQPGIEGVPRRRQDTK

Nucleotide

• Length: 198 bp

>AT114750 NZ_CP033968:141193-141390 [Cupriavidus pauculus]
ATGCGTACGACCATTGCGCTTGACGATGAACTGTTGGCCAAGGCCCAAGCCTATACGGGGCTGGAAGAGAAAACGGCACT
TGTGCGCGAGGCGCTAAAGGCCTTGATCCAGCGTGAAGCCGCCAAACGACTCGCGAACCTTGGCGGGAGCCAACCGGGCA
TCGAAGGGGTACCGCGTCGCCGGCAGGACACCAAATGA

Structures

Toxin

• AlphaFold DB: A0A482J4V6

Antitoxin

• AlphaFold DB: A0A482J603