Detailed information of TA system TA114749

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 3506263..3507180
• Replicon: chromosome
• Accession: NZ_CP033967
• Organism: Bacillus velezensis strain 1B-23

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: EG882_RS17645
• Protein ID: WP_015239694.1
• Coordinates: 3506263..3507009 (+)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: EG882_RS17650
• Protein ID: WP_003154807.1
• Coordinates: 3507010..3507180 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EG882_RS17625	3501649..3502599	-	951	WP_124693083.1	ring-cleaving dioxygenase	-
EG882_RS17630	3502924..3504240	+	1317	WP_007610842.1	amino acid permease	-
EG882_RS17635	3504527..3505144	+	618	WP_003154804.1	DUF47 domain-containing protein	-
EG882_RS17640	3505157..3506155	+	999	WP_007407255.1	inorganic phosphate transporter	-
EG882_RS17645	3506263..3507009	+	747	WP_015239694.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
EG882_RS17650	3507010..3507180	+	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
EG882_RS17655	3507437..3508315	-	879	WP_020955695.1	N-acetylmuramoyl-L-alanine amidase	-
EG882_RS17660	3508329..3508592	-	264	WP_003154813.1	phage holin	-
EG882_RS17665	3508606..3508869	-	264	WP_003154815.1	hemolysin XhlA family protein	-
EG882_RS17670	3508921..3509721	-	801	WP_162992611.1	phage portal protein	-
EG882_RS17675	3509773..3509937	-	165	WP_122503851.1	XkdX family protein	-
EG882_RS17680	3509937..3510263	-	327	WP_122503850.1	XkdW family protein	-
EG882_RS17685	3510275..3512038	-	1764	WP_122503849.1	terminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29135.61 Da
• Isoelectric Point: 4.7003

>T114749 WP_015239694.1 NZ_CP033967:3506263-3507009 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAEWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T114749 NZ_CP033967:3506263-3507009 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTACTGGGCTCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGAATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGAGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCCGCTTATGCCGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCAGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT114749 WP_003154807.1 NZ_CP033967:3507010-3507180 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT114749 NZ_CP033967:3507010-3507180 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Antitoxin

• AlphaFold DB: I2HQ14