Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 148537..149174 Replicon chromosome
Accession NZ_CP033967
Organism Bacillus velezensis strain 1B-23

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag EG882_RS00895 Protein ID WP_003156187.1
Coordinates 148537..148887 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag EG882_RS00900 Protein ID WP_003156188.1
Coordinates 148893..149174 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EG882_RS00855 143577..144179 - 603 WP_007410234.1 SpoIIE family protein phosphatase -
EG882_RS00860 144179..144967 - 789 WP_003156171.1 RNA polymerase sigma factor SigB -
EG882_RS00865 144933..145415 - 483 WP_007609591.1 anti-sigma B factor RsbW -
EG882_RS00870 145412..145741 - 330 WP_033575044.1 anti-sigma factor antagonist -
EG882_RS00875 145805..146812 - 1008 WP_007410233.1 PP2C family protein-serine/threonine phosphatase -
EG882_RS00880 146824..147225 - 402 WP_003156178.1 anti-sigma regulatory factor -
EG882_RS00885 147228..147593 - 366 WP_003156180.1 RsbT antagonist protein RsbS -
EG882_RS00890 147598..148419 - 822 WP_003156182.1 STAS domain-containing protein -
EG882_RS00895 148537..148887 - 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EG882_RS00900 148893..149174 - 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
EG882_RS00905 149294..150463 - 1170 WP_025284272.1 alanine racemase -
EG882_RS00910 150580..151587 - 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
EG882_RS00915 151752..152117 - 366 WP_007410229.1 holo-ACP synthase -
EG882_RS00920 152210..152809 + 600 WP_003156193.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T114748 WP_003156187.1 NZ_CP033967:c148887-148537 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T114748 NZ_CP033967:c148887-148537 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT114748 WP_003156188.1 NZ_CP033967:c149174-148893 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT114748 NZ_CP033967:c149174-148893 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References