Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) pasABC/RelE-HTH
Location 1185..1659 Replicon plasmid p6_L382
Accession NZ_CP033965
Organism Klebsiella pneumoniae strain L482

Toxin (Protein)


Gene name pasB Uniprot ID A0A8J3DT22
Locus tag EHC63_RS30055 Protein ID WP_004197762.1
Coordinates 1393..1659 (+) Length 89 a.a.

Antitoxin (Protein)


Gene name pasA Uniprot ID S1EJF5
Locus tag EHC63_RS30050 Protein ID WP_000879771.1
Coordinates 1185..1409 (+) Length 75 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EHC63_RS30045 306..518 + 213 WP_021527181.1 MbeD family mobilization/exclusion protein -
EHC63_RS30050 1185..1409 + 225 WP_000879771.1 TraY domain-containing protein Antitoxin
EHC63_RS30055 1393..1659 + 267 WP_004197762.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EHC63_RS30060 1729..2079 - 351 WP_004197756.1 hypothetical protein -
EHC63_RS30065 2145..2690 - 546 WP_023317533.1 hypothetical protein -
EHC63_RS30070 2716..3095 - 380 Protein_6 hypothetical protein -
EHC63_RS30745 4198..4374 + 177 WP_004197757.1 hypothetical protein -
EHC63_RS30080 4364..4708 + 345 WP_062955149.1 MobC family plasmid mobilization relaxosome protein -
EHC63_RS30085 4647..5411 + 765 Protein_9 relaxase/mobilization nuclease domain-containing protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..5596 5596


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-87)

Antitoxin

(4-45)


Sequences


Toxin        


Download         Length: 89 a.a.        Molecular weight: 10342.96 Da        Isoelectric Point: 9.8727

>T114738 WP_004197762.1 NZ_CP033965:1393-1659 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Download         Length: 267 bp

>T114738 NZ_CP033965:1393-1659 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8369.28 Da        Isoelectric Point: 4.6533

>AT114738 WP_000879771.1 NZ_CP033965:1185-1409 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Download         Length: 225 bp

>AT114738 NZ_CP033965:1185-1409 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB S1EJF5

References