Detailed information of TA system TA114737

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 6322..6902
• Replicon: plasmid p5_L382
• Accession: NZ_CP033964
• Organism: Klebsiella pneumoniae strain L482

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: EHC63_RS30005
• Protein ID: WP_071177730.1
• Coordinates: 6322..6636 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: EHC63_RS30010
• Protein ID: WP_000093040.1
• Coordinates: 6624..6902 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EHC63_RS29980	2266..4230	+	1965	WP_071177727.1	TraM recognition domain-containing protein	-
EHC63_RS29985	4230..4961	+	732	WP_071177728.1	replication-relaxation family protein	-
EHC63_RS29990	4968..5498	+	531	WP_071177729.1	hypothetical protein	-
EHC63_RS29995	5525..5704	-	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
EHC63_RS30000	5730..6158	-	429	WP_001140599.1	hypothetical protein	-
EHC63_RS30005	6322..6636	-	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EHC63_RS30010	6624..6902	-	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
EHC63_RS30740	7236..7442	-	207	Protein_10	TonB family protein	-
EHC63_RS30020	7439..7810	-	372	WP_001237044.1	cell envelope integrity protein TolA	-
EHC63_RS30025	8084..8329	-	246	WP_032440458.1	hypothetical protein	-
EHC63_RS30035	8974..10461	+	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11970	11970

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T114737 WP_071177730.1 NZ_CP033964:c6636-6322 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T114737 NZ_CP033964:c6636-6322 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT114737 WP_000093040.1 NZ_CP033964:c6902-6624 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT114737 NZ_CP033964:c6902-6624 [Klebsiella pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1