Detailed information of TA system TA114726

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 70388..71058
• Replicon: plasmid p2_L382
• Accession: NZ_CP033961
• Organism: Klebsiella pneumoniae strain L482

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: EHC63_RS27720
• Protein ID: WP_004213072.1
• Coordinates: 70388..70831 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: EHC63_RS27725
• Protein ID: WP_004213073.1
• Coordinates: 70828..71058 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EHC63_RS27670	65795..66070	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
EHC63_RS27675	66133..66624	+	492	WP_011251327.1	DM13 domain-containing protein	-
EHC63_RS27680	66673..67593	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
EHC63_RS27690	67684..68090	+	407	Protein_78	GAF domain-containing protein	-
EHC63_RS30680	68605..69243	-	639	WP_032488580.1	RmpA2	-
EHC63_RS27710	69661..69954	+	294	Protein_80	transposase	-
EHC63_RS27715	69958..70374	+	417	Protein_81	restriction endonuclease	-
EHC63_RS27720	70388..70831	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
EHC63_RS27725	70828..71058	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EHC63_RS27730	71666..72799	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
EHC63_RS27735	72815..73108	+	294	WP_004213076.1	hypothetical protein	-
EHC63_RS27740	73098..73304	-	207	WP_004213077.1	hypothetical protein	-
EHC63_RS27745	73656..73946	+	291	WP_004213078.1	hypothetical protein	-
EHC63_RS27750	73936..74835	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iucA / iucB / iucC / iucD / iutA / rmpA	1..145683	145683

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T114726 WP_004213072.1 NZ_CP033961:c70831-70388 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T114726 NZ_CP033961:c70831-70388 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT114726 WP_004213073.1 NZ_CP033961:c71058-70828 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT114726 NZ_CP033961:c71058-70828 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620