Detailed information of TA system TA114706

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1..475
• Replicon: plasmid p6_L39
• Accession: NZ_CP033959
• Organism: Klebsiella pneumoniae strain L39_2

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: EG820_RS30055
• Protein ID: WP_004197762.1
• Coordinates: 1..267 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: EG820_RS30060
• Protein ID: WP_000879771.1
• Coordinates: 251..475 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EG820_RS30055	1..267	-	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EG820_RS30060	251..475	-	225	WP_000879771.1	TraY domain-containing protein	Antitoxin
EG820_RS30065	1142..1354	-	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
EG820_RS30070	1361..1870	-	510	WP_032433972.1	MbeB family mobilization protein	-
EG820_RS30075	1845..2609	-	765	Protein_4	relaxase/mobilization nuclease domain-containing protein	-
EG820_RS30080	2548..2892	-	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-
EG820_RS30725	2882..3058	-	177	WP_004197757.1	hypothetical protein	-
EG820_RS30090	4161..4540	+	380	Protein_7	hypothetical protein	-
EG820_RS30095	4566..5111	+	546	WP_023317533.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5596	5596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T114706 WP_004197762.1 NZ_CP033959:c267-1 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T114706 NZ_CP033959:c267-1 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT114706 WP_000879771.1 NZ_CP033959:c475-251 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT114706 NZ_CP033959:c475-251 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Antitoxin

• AlphaFold DB: S1EJF5