Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4197827..4198446 Replicon chromosome
Accession NZ_CP033954
Organism Klebsiella pneumoniae strain L39_2

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag EG820_RS21110 Protein ID WP_002892050.1
Coordinates 4198228..4198446 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag EG820_RS21105 Protein ID WP_002892066.1
Coordinates 4197827..4198201 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EG820_RS21095 4192979..4194172 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
EG820_RS21100 4194195..4197341 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit -
EG820_RS21105 4197827..4198201 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
EG820_RS21110 4198228..4198446 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
EG820_RS21115 4198605..4199171 + 567 WP_002892030.1 maltose O-acetyltransferase -
EG820_RS30550 4199143..4199283 - 141 WP_004147370.1 hypothetical protein -
EG820_RS21120 4199304..4199774 + 471 WP_002892026.1 YlaC family protein -
EG820_RS21125 4199749..4201200 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
EG820_RS21130 4201301..4201999 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
EG820_RS21135 4201996..4202136 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
EG820_RS21140 4202136..4202399 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T114691 WP_002892050.1 NZ_CP033954:4198228-4198446 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T114691 NZ_CP033954:4198228-4198446 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT114691 WP_002892066.1 NZ_CP033954:4197827-4198201 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT114691 NZ_CP033954:4197827-4198201 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References