Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 52426..52951 Replicon plasmid p2
Accession NZ_CP033948
Organism Klebsiella pneumoniae subsp. pneumoniae strain ARLG-3135

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag EG819_RS27670 Protein ID WP_001159868.1
Coordinates 52646..52951 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag EG819_RS27665 Protein ID WP_000813634.1
Coordinates 52426..52644 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EG819_RS27635 47819..48235 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
EG819_RS27640 48232..48462 - 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
EG819_RS27645 48727..49227 + 501 WP_000528931.1 hypothetical protein -
EG819_RS27650 49240..50013 + 774 WP_000905949.1 hypothetical protein -
EG819_RS27655 50180..51313 + 1134 WP_000545987.1 DUF3800 domain-containing protein -
EG819_RS27660 51347..51859 - 513 WP_000151784.1 hypothetical protein -
EG819_RS27665 52426..52644 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
EG819_RS27670 52646..52951 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
EG819_RS27675 52952..53758 + 807 WP_000016982.1 site-specific integrase -
EG819_RS27680 54532..55287 + 756 WP_000852146.1 replication initiation protein RepE -
EG819_RS27685 55875..57041 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) / mph(A) / sul1 / qacE / aadA2 / dfrA12 / sitABCD iucA / iucB / iucC / iucD / iutA 1..70586 70586


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T114677 WP_001159868.1 NZ_CP033948:52646-52951 [Klebsiella pneumoniae subsp. pneumoniae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T114677 NZ_CP033948:52646-52951 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT114677 WP_000813634.1 NZ_CP033948:52426-52644 [Klebsiella pneumoniae subsp. pneumoniae]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT114677 NZ_CP033948:52426-52644 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References