Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 47819..48462 Replicon plasmid p2
Accession NZ_CP033948
Organism Klebsiella pneumoniae subsp. pneumoniae strain ARLG-3135

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag EG819_RS27635 Protein ID WP_001034046.1
Coordinates 47819..48235 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag EG819_RS27640 Protein ID WP_001261278.1
Coordinates 48232..48462 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EG819_RS27620 42956..43372 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
EG819_RS27625 43369..43599 - 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
EG819_RS27630 43980..47774 + 3795 WP_001144732.1 hypothetical protein -
EG819_RS27635 47819..48235 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
EG819_RS27640 48232..48462 - 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
EG819_RS27645 48727..49227 + 501 WP_000528931.1 hypothetical protein -
EG819_RS27650 49240..50013 + 774 WP_000905949.1 hypothetical protein -
EG819_RS27655 50180..51313 + 1134 WP_000545987.1 DUF3800 domain-containing protein -
EG819_RS27660 51347..51859 - 513 WP_000151784.1 hypothetical protein -
EG819_RS27665 52426..52644 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
EG819_RS27670 52646..52951 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) / mph(A) / sul1 / qacE / aadA2 / dfrA12 / sitABCD iucA / iucB / iucC / iucD / iutA 1..70586 70586


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T114676 WP_001034046.1 NZ_CP033948:c48235-47819 [Klebsiella pneumoniae subsp. pneumoniae]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T114676 NZ_CP033948:c48235-47819 [Klebsiella pneumoniae subsp. pneumoniae]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT114676 WP_001261278.1 NZ_CP033948:c48462-48232 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT114676 NZ_CP033948:c48462-48232 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References