Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4031368..4031987 Replicon chromosome
Accession NZ_CP033946
Organism Klebsiella pneumoniae subsp. pneumoniae strain ARLG-3135

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag EG819_RS20045 Protein ID WP_002892050.1
Coordinates 4031769..4031987 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag EG819_RS20040 Protein ID WP_002892066.1
Coordinates 4031368..4031742 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EG819_RS20030 4026520..4027713 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
EG819_RS20035 4027736..4030882 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
EG819_RS20040 4031368..4031742 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
EG819_RS20045 4031769..4031987 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
EG819_RS20050 4032150..4032716 + 567 WP_002892030.1 maltose O-acetyltransferase -
EG819_RS28770 4032688..4032828 - 141 WP_004147370.1 hypothetical protein -
EG819_RS20055 4032849..4033319 + 471 WP_002892026.1 YlaC family protein -
EG819_RS20060 4033294..4034745 - 1452 WP_064162152.1 PLP-dependent aminotransferase family protein -
EG819_RS20065 4034846..4035544 + 699 WP_004177238.1 GNAT family N-acetyltransferase -
EG819_RS20070 4035541..4035681 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
EG819_RS20075 4035681..4035944 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T114671 WP_002892050.1 NZ_CP033946:4031769-4031987 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T114671 NZ_CP033946:4031769-4031987 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT114671 WP_002892066.1 NZ_CP033946:4031368-4031742 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT114671 NZ_CP033946:4031368-4031742 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References