Detailed information of TA system TA114658

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 21016..21529
• Replicon: plasmid pPSEM90-8
• Accession: NZ_CP033945
• Organism: Piscirickettsia salmonis strain EM-90

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: EG881_RS19955
• Protein ID: WP_016211076.1
• Coordinates: 21362..21529 (-)
• Length: 56 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A7U4WZG4
• Locus tag: EG881_RS19950
• Protein ID: WP_016211078.1
• Coordinates: 21016..21369 (-)
• Length: 118 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EG881_RS19925	16451..17260	-	810	WP_129556746.1	queuosine precursor transporter	-
EG881_RS19930	17244..17675	-	432	WP_016211069.1	hypothetical protein	-
EG881_RS20755	17700..17843	-	144	WP_016211073.1	hypothetical protein	-
EG881_RS19935	18058..19299	+	1242	WP_016211075.1	MFS transporter	-
EG881_RS19940	19303..20115	+	813	WP_016211068.1	helix-turn-helix transcriptional regulator	-
EG881_RS19945	20112..20912	-	801	WP_016211074.1	methyltransferase domain-containing protein	-
EG881_RS19950	21016..21369	-	354	WP_016211078.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EG881_RS19955	21362..21529	-	168	WP_016211076.1	type II toxin-antitoxin system HicA family toxin	Toxin
EG881_RS19960	21553..22527	-	975	WP_054300271.1	IS30 family transposase	-
EG881_RS19965	22953..23282	-	330	WP_016211926.1	hypothetical protein	-
EG881_RS19970	23738..24523	+	786	WP_016211925.1	ParA family protein	-
EG881_RS19975	24516..24956	+	441	WP_016211928.1	hypothetical protein	-
EG881_RS19980	25507..25770	+	264	WP_032126138.1	RNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..36078	36078

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-51)

Antitoxin

• Uncharacterised protein family HicB (53-103)

Sequences

Toxin

Protein

• Length: 56 a.a.
• Molecular weight: 6104.00 Da
• Isoelectric Point: 10.4868

>T114658 WP_016211076.1 NZ_CP033945:c21529-21362 [Piscirickettsia salmonis]
MTEGEVIERDGSRVAVILNQVKTTFHRPHPEKTTDKGAVKSIRLFLKNAGVDKNA

Nucleotide

• Length: 168 bp

>T114658 NZ_CP033945:c21529-21362 [Piscirickettsia salmonis]
ATGACGGAGGGCGAAGTAATAGAGCGTGATGGTTCAAGGGTTGCTGTAATTTTGAATCAAGTGAAAACAACATTTCATAG
ACCGCACCCTGAAAAAACAACAGACAAGGGGGCGGTTAAGTCAATAAGGTTGTTTTTAAAAAATGCTGGAGTAGATAAAA
ATGCTTAA

Antitoxin

Protein

• Length: 118 a.a.
• Molecular weight: 13492.39 Da
• Isoelectric Point: 5.0200

>AT114658 WP_016211078.1 NZ_CP033945:c21369-21016 [Piscirickettsia salmonis]
MLKYKGYIGKVEEIHEGDEYIYGIVPNIKDVISFRGKNIKELTKDFKLAIDDYLALCDEREEEPDKPFSGRFMTRLGSDL
HEEVFLASMNDSGAKSMNDWVVSACLEKLKHDQTVKR

Nucleotide

• Length: 354 bp

>AT114658 NZ_CP033945:c21369-21016 [Piscirickettsia salmonis]
ATGCTTAAATACAAAGGTTATATTGGAAAAGTTGAAGAGATTCATGAAGGTGATGAATATATTTATGGGATTGTTCCAAA
TATTAAAGATGTTATTTCTTTTAGAGGTAAAAATATAAAAGAGTTAACGAAAGACTTTAAGCTGGCTATTGATGATTATT
TGGCTTTATGTGATGAGCGTGAAGAAGAACCCGATAAACCATTTTCTGGTAGATTTATGACTAGATTAGGTAGTGATTTG
CATGAAGAGGTTTTTTTAGCTTCTATGAATGATTCAGGTGCAAAATCAATGAATGATTGGGTTGTATCTGCATGCTTGGA
AAAGTTAAAGCATGATCAAACCGTTAAACGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U4WZG4