Detailed information of TA system TA114649

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3574953..3575517
• Replicon: chromosome
• Accession: NZ_CP033911
• Organism: Mycobacterium avium subsp. paratuberculosis strain MAPK_JB16/15

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: X7VBD8
• Locus tag: EGM64_RS16920
• Protein ID: WP_003875391.1
• Coordinates: 3574953..3575324 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7L5MGM2
• Locus tag: EGM64_RS16925
• Protein ID: WP_003875390.1
• Coordinates: 3575311..3575517 (-)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGM64_RS16875	3570290..3570487	-	198	WP_016706054.1	hypothetical protein	-
EGM64_RS16880	3570605..3571288	+	684	WP_003875399.1	hypothetical protein	-
EGM64_RS16885	3571293..3571617	-	325	Protein_3282	antibiotic biosynthesis monooxygenase	-
EGM64_RS16890	3572028..3572552	-	525	WP_033713819.1	glycoside hydrolase	-
EGM64_RS16895	3572709..3573062	-	354	WP_003875396.1	hypothetical protein	-
EGM64_RS16900	3573132..3573515	-	384	WP_003875395.1	hypothetical protein	-
EGM64_RS16905	3573582..3573980	-	399	WP_003875394.1	VOC family protein	-
EGM64_RS16910	3574082..3574357	-	276	WP_003878531.1	hypothetical protein	-
EGM64_RS16915	3574413..3574853	-	441	WP_010949694.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
EGM64_RS16920	3574953..3575324	-	372	WP_003875391.1	type II toxin-antitoxin system VapC family toxin	Toxin
EGM64_RS16925	3575311..3575517	-	207	WP_003875390.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
EGM64_RS16930	3575686..3576630	-	945	WP_003875389.1	HNH endonuclease	-
EGM64_RS16935	3576830..3577903	-	1074	WP_003878533.1	redox-regulated ATPase YchF	-
EGM64_RS16940	3578034..3579242	+	1209	WP_010949696.1	hypothetical protein	-
EGM64_RS16945	3579266..3580264	-	999	WP_003875386.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13770.14 Da
• Isoelectric Point: 6.6003

>T114649 WP_003875391.1 NZ_CP033911:c3575324-3574953 [Mycobacterium avium subsp. paratuberculosis]
MILVDTSVWIDHLHVADKRLIEFLTNDAIGCHQMVIEELALGAIRRRADLLRLLSNLRAFPLLTHAEVLHLVECHRLWGR
GLSAIDVHLLGSVALVDGARLWTRDKNLKAAGWDVGIAIVDEL

Nucleotide

• Length: 372 bp

>T114649 NZ_CP033911:c3575324-3574953 [Mycobacterium avium subsp. paratuberculosis]
ATGATTCTCGTTGACACGTCGGTATGGATCGATCATCTGCACGTCGCCGACAAGCGACTCATAGAGTTCCTGACCAACGA
CGCAATCGGTTGCCACCAAATGGTGATCGAGGAGTTGGCATTGGGTGCGATCAGGCGGCGCGCCGATCTATTGCGCCTGC
TGTCCAATCTTCGGGCGTTCCCGTTGTTGACGCATGCGGAGGTCCTTCATCTCGTCGAATGCCACCGCTTGTGGGGCCGC
GGACTGAGTGCGATCGACGTCCATCTTCTCGGGTCGGTTGCTCTGGTCGACGGTGCACGGCTATGGACCAGGGACAAGAA
TTTAAAAGCGGCGGGCTGGGACGTGGGTATCGCGATCGTCGACGAGCTCTGA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7231.22 Da
• Isoelectric Point: 11.0634

>AT114649 WP_003875390.1 NZ_CP033911:c3575517-3575311 [Mycobacterium avium subsp. paratuberculosis]
MRTTVTIDDALLAEAAELTGVTESVALLRQGLQTLVRVESARRLAALGGTDRKASVGPRRRAPADDSR

Nucleotide

• Length: 207 bp

>AT114649 NZ_CP033911:c3575517-3575311 [Mycobacterium avium subsp. paratuberculosis]
ATGAGGACGACGGTGACGATTGACGACGCCTTATTGGCAGAGGCGGCCGAACTGACCGGGGTGACAGAGAGCGTGGCGCT
CCTCCGTCAGGGACTGCAGACACTCGTTCGGGTAGAGAGCGCCCGTCGGCTGGCGGCGCTCGGCGGCACGGATCGGAAGG
CATCGGTCGGCCCTCGCCGGAGGGCACCCGCCGATGATTCTCGTTGA

Structures

Toxin

• AlphaFold DB: A0A0E2WB37

Antitoxin

• AlphaFold DB: A0A7L5MGM2