Detailed information of TA system TA114648

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-PHD
• Location: 2794439..2795103
• Replicon: chromosome
• Accession: NZ_CP033911
• Organism: Mycobacterium avium subsp. paratuberculosis strain MAPK_JB16/15

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q73YC9
• Locus tag: EGM64_RS13475
• Protein ID: WP_003872398.1
• Coordinates: 2794439..2794846 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q73YC8
• Locus tag: EGM64_RS13480
• Protein ID: WP_003872399.1
• Coordinates: 2794843..2795103 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGM64_RS13450	2789639..2790808	+	1170	WP_003878212.1	hypothetical protein	-
EGM64_RS13455	2790797..2791327	-	531	WP_003878213.1	TetR/AcrR family transcriptional regulator	-
EGM64_RS13460	2791448..2791993	-	546	WP_003878214.1	carboxymuconolactone decarboxylase family protein	-
EGM64_RS13465	2791990..2792511	-	522	WP_003878215.1	carboxymuconolactone decarboxylase family protein	-
EGM64_RS13470	2792748..2794292	+	1545	WP_010949452.1	serine/threonine protein kinase	-
EGM64_RS13475	2794439..2794846	-	408	WP_003872398.1	type II toxin-antitoxin system VapC family toxin	Toxin
EGM64_RS13480	2794843..2795103	-	261	WP_003872399.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
EGM64_RS13485	2795284..2795736	-	453	WP_003872400.1	VOC family protein	-
EGM64_RS13490	2795902..2797170	+	1269	WP_003872401.1	cytochrome P450	-
EGM64_RS13495	2797372..2798829	-	1458	WP_003878220.1	serine/threonine protein kinase	-
EGM64_RS13500	2799025..2799687	-	663	WP_003872403.1	TetR/AcrR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-125)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-64)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14418.47 Da
• Isoelectric Point: 4.5642

>T114648 WP_003872398.1 NZ_CP033911:c2794846-2794439 [Mycobacterium avium subsp. paratuberculosis]
MTARPAAGVLDTSVFIASESGRQLDEARIPDEVATTVVTLAELHAGVLAATTSNVRAQRLATLESIADMEMLPVDDDAVR
MWARLRIHLAEAGRRMRANDLWIAAIAASRGLPVVTQDDDFAALDGAANLAIIRV

Nucleotide

• Length: 408 bp

>T114648 NZ_CP033911:c2794846-2794439 [Mycobacterium avium subsp. paratuberculosis]
ATGACTGCAAGGCCGGCGGCCGGGGTGCTCGACACCTCGGTGTTCATCGCGTCCGAAAGCGGACGGCAGCTGGACGAGGC
GCGTATCCCCGACGAGGTAGCCACCACCGTCGTCACCCTTGCCGAACTTCACGCCGGCGTATTAGCCGCGACGACGTCCA
ACGTTCGGGCGCAACGCCTCGCAACGCTGGAATCGATAGCGGATATGGAAATGCTGCCAGTCGACGACGACGCGGTCCGC
ATGTGGGCAAGGTTGCGGATCCACCTAGCCGAGGCTGGACGGCGGATGCGGGCTAATGATCTGTGGATCGCGGCGATCGC
GGCATCGCGGGGACTGCCCGTTGTCACCCAGGATGACGACTTCGCCGCACTCGACGGCGCGGCGAACCTGGCGATTATTC
GGGTTTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9635.01 Da
• Isoelectric Point: 11.7137

>AT114648 WP_003872399.1 NZ_CP033911:c2795103-2794843 [Mycobacterium avium subsp. paratuberculosis]
MSEVASRELRNDTAGVLRRVREGEDVTITINGRAVAMLTPLRPQRRRWLSKAELLSRLRRAQADPGLRQDLATLAGDTTE
DLGPIR

Nucleotide

• Length: 261 bp

>AT114648 NZ_CP033911:c2795103-2794843 [Mycobacterium avium subsp. paratuberculosis]
ATGTCTGAAGTGGCGTCTCGCGAGCTGCGCAACGATACGGCGGGGGTACTGCGGCGCGTACGGGAAGGGGAGGACGTCAC
CATCACCATCAACGGCCGAGCCGTCGCGATGCTGACCCCGCTGCGGCCGCAGCGACGGCGGTGGCTGAGCAAGGCGGAGT
TGCTGTCGCGCTTGCGCCGCGCGCAAGCCGATCCCGGGCTACGCCAAGACCTCGCAACCCTTGCAGGCGACACCACTGAG
GACCTCGGTCCGATCCGATGA

Structures

Toxin

• AlphaFold DB: A0A7L5MIC8

Antitoxin

• AlphaFold DB: A0A7L5MIC5