Detailed information of TA system TA114646

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1418153..1418806
• Replicon: chromosome
• Accession: NZ_CP033911
• Organism: Mycobacterium avium subsp. paratuberculosis strain MAPK_JB16/15

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: EGM64_RS07250
• Protein ID: WP_003872880.1
• Coordinates: 1418345..1418806 (+)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: Q742J1
• Locus tag: EGM64_RS07245
• Protein ID: WP_003872881.1
• Coordinates: 1418153..1418341 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGM64_RS07235	1414135..1415721	+	1587	WP_003872883.1	serine hydrolase	-
EGM64_RS07240	1415718..1418090	+	2373	WP_003872882.1	HAD-IC family P-type ATPase	-
EGM64_RS07245	1418153..1418341	+	189	WP_003872881.1	antitoxin	Antitoxin
EGM64_RS07250	1418345..1418806	+	462	WP_003872880.1	SRPBCC family protein	Toxin
EGM64_RS07255	1418875..1419309	+	435	WP_003877359.1	hypothetical protein	-
EGM64_RS07260	1420305..1421888	+	1584	WP_003872878.1	DUF4185 domain-containing protein	-
EGM64_RS07265	1421928..1422314	+	387	WP_003872877.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (2-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-51)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16626.19 Da
• Isoelectric Point: 9.5654

>T114646 WP_003872880.1 NZ_CP033911:1418345-1418806 [Mycobacterium avium subsp. paratuberculosis]
VAKLSGSIDVPLPPEVAWQHASDLSRYKEWLTIHRVWRSTLPDQIDKGTVVESIVEVKGMLNRIKWTVVRYKPPEGMTLN
GDGVGGVKVKLMAKVQPKADGSVVSFDVHLGGPALFGPIGMIVAAALRGDIDQSLENFVTVFARSDPSTNGHR

Nucleotide

• Length: 462 bp

>T114646 NZ_CP033911:1418345-1418806 [Mycobacterium avium subsp. paratuberculosis]
GTGGCCAAGCTTTCCGGATCCATCGATGTTCCGCTGCCCCCCGAGGTGGCCTGGCAGCACGCCTCCGACCTGTCCCGGTA
CAAGGAGTGGCTGACCATTCACCGGGTGTGGCGCAGCACCTTGCCCGACCAGATCGACAAGGGCACCGTCGTCGAGTCGA
TCGTCGAGGTCAAAGGCATGCTCAACCGGATCAAATGGACGGTGGTGCGCTACAAGCCACCGGAGGGGATGACGCTCAAC
GGCGACGGCGTCGGCGGGGTCAAGGTCAAGCTGATGGCCAAGGTGCAGCCCAAGGCGGACGGTTCCGTCGTCAGCTTCGA
CGTGCACCTCGGCGGTCCGGCGCTGTTCGGGCCCATCGGCATGATCGTCGCGGCCGCGCTGCGCGGCGACATCGACCAGT
CGCTGGAGAACTTCGTCACGGTGTTCGCCCGATCGGATCCGAGCACCAACGGTCATCGCTGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6745.42 Da
• Isoelectric Point: 4.4865

>AT114646 WP_003872881.1 NZ_CP033911:1418153-1418341 [Mycobacterium avium subsp. paratuberculosis]
MGFLDKAKDLLSQNADKVEMAIDKAGEFVDEKTQGKYSDTIHQVQEQAKKATGATETGDQQG

Nucleotide

• Length: 189 bp

>AT114646 NZ_CP033911:1418153-1418341 [Mycobacterium avium subsp. paratuberculosis]
ATGGGATTCCTGGACAAGGCCAAGGACCTGTTGTCGCAGAACGCCGACAAGGTCGAGATGGCCATCGACAAGGCCGGCGA
GTTCGTCGACGAGAAGACCCAGGGCAAGTACTCCGACACCATCCACCAGGTGCAGGAGCAGGCCAAGAAGGCCACCGGCG
CCACCGAGACCGGCGACCAGCAGGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0E2WC18