Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-PHD
Location 3971026..3971690 Replicon chromosome
Accession NZ_CP033910
Organism Mycobacterium avium subsp. paratuberculosis strain MAPK_CN4/13

Toxin (Protein)


Gene name vapC Uniprot ID Q73YC9
Locus tag EGM63_RS19170 Protein ID WP_003872398.1
Coordinates 3971283..3971690 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q73YC8
Locus tag EGM63_RS19165 Protein ID WP_003872399.1
Coordinates 3971026..3971286 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGM63_RS19145 3966442..3967104 + 663 WP_003872403.1 TetR/AcrR family transcriptional regulator -
EGM63_RS19150 3967300..3968757 + 1458 WP_003878220.1 serine/threonine protein kinase -
EGM63_RS19155 3968959..3970227 - 1269 WP_003872401.1 cytochrome P450 -
EGM63_RS19160 3970393..3970845 + 453 WP_003872400.1 VOC family protein -
EGM63_RS19165 3971026..3971286 + 261 WP_003872399.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EGM63_RS19170 3971283..3971690 + 408 WP_003872398.1 type II toxin-antitoxin system VapC family toxin Toxin
EGM63_RS19175 3971837..3973381 - 1545 WP_010949452.1 serine/threonine protein kinase -
EGM63_RS19180 3973618..3974139 + 522 WP_003878215.1 carboxymuconolactone decarboxylase family protein -
EGM63_RS19185 3974136..3974681 + 546 WP_003878214.1 carboxymuconolactone decarboxylase family protein -
EGM63_RS19190 3974802..3975332 + 531 WP_003878213.1 TetR/AcrR family transcriptional regulator -
EGM63_RS19195 3975321..3976490 - 1170 WP_003878212.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(9-125)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-64)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 14418.47 Da        Isoelectric Point: 4.5642

>T114643 WP_003872398.1 NZ_CP033910:3971283-3971690 [Mycobacterium avium subsp. paratuberculosis]
MTARPAAGVLDTSVFIASESGRQLDEARIPDEVATTVVTLAELHAGVLAATTSNVRAQRLATLESIADMEMLPVDDDAVR
MWARLRIHLAEAGRRMRANDLWIAAIAASRGLPVVTQDDDFAALDGAANLAIIRV

Download         Length: 408 bp

>T114643 NZ_CP033910:3971283-3971690 [Mycobacterium avium subsp. paratuberculosis]
ATGACTGCAAGGCCGGCGGCCGGGGTGCTCGACACCTCGGTGTTCATCGCGTCCGAAAGCGGACGGCAGCTGGACGAGGC
GCGTATCCCCGACGAGGTAGCCACCACCGTCGTCACCCTTGCCGAACTTCACGCCGGCGTATTAGCCGCGACGACGTCCA
ACGTTCGGGCGCAACGCCTCGCAACGCTGGAATCGATAGCGGATATGGAAATGCTGCCAGTCGACGACGACGCGGTCCGC
ATGTGGGCAAGGTTGCGGATCCACCTAGCCGAGGCTGGACGGCGGATGCGGGCTAATGATCTGTGGATCGCGGCGATCGC
GGCATCGCGGGGACTGCCCGTTGTCACCCAGGATGACGACTTCGCCGCACTCGACGGCGCGGCGAACCTGGCGATTATTC
GGGTTTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9635.01 Da        Isoelectric Point: 11.7137

>AT114643 WP_003872399.1 NZ_CP033910:3971026-3971286 [Mycobacterium avium subsp. paratuberculosis]
MSEVASRELRNDTAGVLRRVREGEDVTITINGRAVAMLTPLRPQRRRWLSKAELLSRLRRAQADPGLRQDLATLAGDTTE
DLGPIR

Download         Length: 261 bp

>AT114643 NZ_CP033910:3971026-3971286 [Mycobacterium avium subsp. paratuberculosis]
ATGTCTGAAGTGGCGTCTCGCGAGCTGCGCAACGATACGGCGGGGGTACTGCGGCGCGTACGGGAAGGGGAGGACGTCAC
CATCACCATCAACGGCCGAGCCGTCGCGATGCTGACCCCGCTGCGGCCGCAGCGACGGCGGTGGCTGAGCAAGGCGGAGT
TGCTGTCGCGCTTGCGCCGCGCGCAAGCCGATCCCGGGCTACGCCAAGACCTCGCAACCCTTGCAGGCGACACCACTGAG
GACCTCGGTCCGATCCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L5MIC8

Antitoxin

Source ID Structure
AlphaFold DB A0A7L5MIC5

References