Detailed information of TA system TA114642

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3192065..3192629
• Replicon: chromosome
• Accession: NZ_CP033910
• Organism: Mycobacterium avium subsp. paratuberculosis strain MAPK_CN4/13

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: X7VBD8
• Locus tag: EGM63_RS15735
• Protein ID: WP_003875391.1
• Coordinates: 3192258..3192629 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7L5MGM2
• Locus tag: EGM63_RS15730
• Protein ID: WP_003875390.1
• Coordinates: 3192065..3192271 (+)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGM63_RS15710	3187318..3188316	+	999	WP_003875386.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
EGM63_RS15715	3188340..3189548	-	1209	WP_010949696.1	hypothetical protein	-
EGM63_RS15720	3189679..3190752	+	1074	WP_003878533.1	redox-regulated ATPase YchF	-
EGM63_RS15725	3190952..3191896	+	945	WP_003875389.1	HNH endonuclease	-
EGM63_RS15730	3192065..3192271	+	207	WP_003875390.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
EGM63_RS15735	3192258..3192629	+	372	WP_003875391.1	type II toxin-antitoxin system VapC family toxin	Toxin
EGM63_RS15740	3192729..3193169	+	441	WP_010949694.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
EGM63_RS15745	3193225..3193500	+	276	WP_003878531.1	hypothetical protein	-
EGM63_RS15750	3193602..3194000	+	399	WP_003875394.1	VOC family protein	-
EGM63_RS15755	3194067..3194450	+	384	WP_003875395.1	hypothetical protein	-
EGM63_RS15760	3194520..3194873	+	354	WP_003875396.1	hypothetical protein	-
EGM63_RS15765	3195030..3195554	+	525	WP_033713819.1	glycoside hydrolase	-
EGM63_RS15770	3195965..3196289	+	325	Protein_3066	antibiotic biosynthesis monooxygenase	-
EGM63_RS15775	3196294..3196977	-	684	WP_003875399.1	hypothetical protein	-
EGM63_RS15780	3197095..3197292	+	198	WP_016706054.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-112)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13770.14 Da
• Isoelectric Point: 6.6003

>T114642 WP_003875391.1 NZ_CP033910:3192258-3192629 [Mycobacterium avium subsp. paratuberculosis]
MILVDTSVWIDHLHVADKRLIEFLTNDAIGCHQMVIEELALGAIRRRADLLRLLSNLRAFPLLTHAEVLHLVECHRLWGR
GLSAIDVHLLGSVALVDGARLWTRDKNLKAAGWDVGIAIVDEL

Nucleotide

• Length: 372 bp

>T114642 NZ_CP033910:3192258-3192629 [Mycobacterium avium subsp. paratuberculosis]
ATGATTCTCGTTGACACGTCGGTATGGATCGATCATCTGCACGTCGCCGACAAGCGACTCATAGAGTTCCTGACCAACGA
CGCAATCGGTTGCCACCAAATGGTGATCGAGGAGTTGGCATTGGGTGCGATCAGGCGGCGCGCCGATCTATTGCGCCTGC
TGTCCAATCTTCGGGCGTTCCCGTTGTTGACGCATGCGGAGGTCCTTCATCTCGTCGAATGCCACCGCTTGTGGGGCCGC
GGACTGAGTGCGATCGACGTCCATCTTCTCGGGTCGGTTGCTCTGGTCGACGGTGCACGGCTATGGACCAGGGACAAGAA
TTTAAAAGCGGCGGGCTGGGACGTGGGTATCGCGATCGTCGACGAGCTCTGA

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7231.22 Da
• Isoelectric Point: 11.0634

>AT114642 WP_003875390.1 NZ_CP033910:3192065-3192271 [Mycobacterium avium subsp. paratuberculosis]
MRTTVTIDDALLAEAAELTGVTESVALLRQGLQTLVRVESARRLAALGGTDRKASVGPRRRAPADDSR

Nucleotide

• Length: 207 bp

>AT114642 NZ_CP033910:3192065-3192271 [Mycobacterium avium subsp. paratuberculosis]
ATGAGGACGACGGTGACGATTGACGACGCCTTATTGGCAGAGGCGGCCGAACTGACCGGGGTGACAGAGAGCGTGGCGCT
CCTCCGTCAGGGACTGCAGACACTCGTTCGGGTAGAGAGCGCCCGTCGGCTGGCGGCGCTCGGCGGCACGGATCGGAAGG
CATCGGTCGGCCCTCGCCGGAGGGCACCCGCCGATGATTCTCGTTGA

Structures

Toxin

• AlphaFold DB: A0A0E2WB37

Antitoxin

• AlphaFold DB: A0A7L5MGM2