Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 2962230..2962794 Replicon chromosome
Accession NZ_CP033909
Organism Mycobacterium avium subsp. paratuberculosis strain MAPK_JJ1/13

Toxin (Protein)


Gene name vapC Uniprot ID X7VBD8
Locus tag EGM60_RS13820 Protein ID WP_003875391.1
Coordinates 2962230..2962601 (-) Length 124 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A7L5MGM2
Locus tag EGM60_RS13825 Protein ID WP_003875390.1
Coordinates 2962588..2962794 (-) Length 69 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGM60_RS13775 2957567..2957764 - 198 WP_016706054.1 hypothetical protein -
EGM60_RS13780 2957882..2958565 + 684 WP_003875399.1 hypothetical protein -
EGM60_RS13785 2958570..2958894 - 325 Protein_2681 antibiotic biosynthesis monooxygenase -
EGM60_RS13790 2959305..2959829 - 525 WP_033713819.1 glycoside hydrolase -
EGM60_RS13795 2959986..2960339 - 354 WP_003875396.1 hypothetical protein -
EGM60_RS13800 2960409..2960792 - 384 WP_003875395.1 hypothetical protein -
EGM60_RS13805 2960859..2961257 - 399 WP_003875394.1 VOC family protein -
EGM60_RS13810 2961359..2961634 - 276 WP_003878531.1 hypothetical protein -
EGM60_RS13815 2961690..2962130 - 441 WP_010949694.1 nitroreductase family deazaflavin-dependent oxidoreductase -
EGM60_RS13820 2962230..2962601 - 372 WP_003875391.1 type II toxin-antitoxin system VapC family toxin Toxin
EGM60_RS13825 2962588..2962794 - 207 WP_003875390.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
EGM60_RS13830 2962963..2963907 - 945 WP_003875389.1 HNH endonuclease -
EGM60_RS13835 2964107..2965180 - 1074 WP_003878533.1 redox-regulated ATPase YchF -
EGM60_RS13840 2965311..2966519 + 1209 WP_010949696.1 hypothetical protein -
EGM60_RS13845 2966543..2967541 - 999 WP_003875386.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-112)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-42)


Sequences

Toxin        


Download         Length: 124 a.a.        Molecular weight: 13770.14 Da        Isoelectric Point: 6.6003

>T114638 WP_003875391.1 NZ_CP033909:c2962601-2962230 [Mycobacterium avium subsp. paratuberculosis]
MILVDTSVWIDHLHVADKRLIEFLTNDAIGCHQMVIEELALGAIRRRADLLRLLSNLRAFPLLTHAEVLHLVECHRLWGR
GLSAIDVHLLGSVALVDGARLWTRDKNLKAAGWDVGIAIVDEL

Download         Length: 372 bp

>T114638 NZ_CP033909:c2962601-2962230 [Mycobacterium avium subsp. paratuberculosis]
ATGATTCTCGTTGACACGTCGGTATGGATCGATCATCTGCACGTCGCCGACAAGCGACTCATAGAGTTCCTGACCAACGA
CGCAATCGGTTGCCACCAAATGGTGATCGAGGAGTTGGCATTGGGTGCGATCAGGCGGCGCGCCGATCTATTGCGCCTGC
TGTCCAATCTTCGGGCGTTCCCGTTGTTGACGCATGCGGAGGTCCTTCATCTCGTCGAATGCCACCGCTTGTGGGGCCGC
GGACTGAGTGCGATCGACGTCCATCTTCTCGGGTCGGTTGCTCTGGTCGACGGTGCACGGCTATGGACCAGGGACAAGAA
TTTAAAAGCGGCGGGCTGGGACGTGGGTATCGCGATCGTCGACGAGCTCTGA

Antitoxin


Download         Length: 69 a.a.        Molecular weight: 7231.22 Da        Isoelectric Point: 11.0634

>AT114638 WP_003875390.1 NZ_CP033909:c2962794-2962588 [Mycobacterium avium subsp. paratuberculosis]
MRTTVTIDDALLAEAAELTGVTESVALLRQGLQTLVRVESARRLAALGGTDRKASVGPRRRAPADDSR

Download         Length: 207 bp

>AT114638 NZ_CP033909:c2962794-2962588 [Mycobacterium avium subsp. paratuberculosis]
ATGAGGACGACGGTGACGATTGACGACGCCTTATTGGCAGAGGCGGCCGAACTGACCGGGGTGACAGAGAGCGTGGCGCT
CCTCCGTCAGGGACTGCAGACACTCGTTCGGGTAGAGAGCGCCCGTCGGCTGGCGGCGCTCGGCGGCACGGATCGGAAGG
CATCGGTCGGCCCTCGCCGGAGGGCACCCGCCGATGATTCTCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E2WB37

Antitoxin

Source ID Structure
AlphaFold DB A0A7L5MGM2

References