Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-PHD
Location 2181746..2182410 Replicon chromosome
Accession NZ_CP033909
Organism Mycobacterium avium subsp. paratuberculosis strain MAPK_JJ1/13

Toxin (Protein)


Gene name vapC Uniprot ID Q73YC9
Locus tag EGM60_RS10370 Protein ID WP_003872398.1
Coordinates 2181746..2182153 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q73YC8
Locus tag EGM60_RS10375 Protein ID WP_003872399.1
Coordinates 2182150..2182410 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGM60_RS10345 2176946..2178115 + 1170 WP_003878212.1 hypothetical protein -
EGM60_RS10350 2178104..2178634 - 531 WP_003878213.1 TetR/AcrR family transcriptional regulator -
EGM60_RS10355 2178755..2179300 - 546 WP_003878214.1 carboxymuconolactone decarboxylase family protein -
EGM60_RS10360 2179297..2179818 - 522 WP_003878215.1 carboxymuconolactone decarboxylase family protein -
EGM60_RS10365 2180055..2181599 + 1545 WP_010949452.1 serine/threonine protein kinase -
EGM60_RS10370 2181746..2182153 - 408 WP_003872398.1 type II toxin-antitoxin system VapC family toxin Toxin
EGM60_RS10375 2182150..2182410 - 261 WP_003872399.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EGM60_RS10380 2182591..2183043 - 453 WP_003872400.1 VOC family protein -
EGM60_RS10385 2183209..2184477 + 1269 WP_003872401.1 cytochrome P450 -
EGM60_RS10390 2184679..2186136 - 1458 WP_003878220.1 serine/threonine protein kinase -
EGM60_RS10395 2186332..2186994 - 663 WP_003872403.1 TetR/AcrR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(9-125)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-64)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 14418.47 Da        Isoelectric Point: 4.5642

>T114637 WP_003872398.1 NZ_CP033909:c2182153-2181746 [Mycobacterium avium subsp. paratuberculosis]
MTARPAAGVLDTSVFIASESGRQLDEARIPDEVATTVVTLAELHAGVLAATTSNVRAQRLATLESIADMEMLPVDDDAVR
MWARLRIHLAEAGRRMRANDLWIAAIAASRGLPVVTQDDDFAALDGAANLAIIRV

Download         Length: 408 bp

>T114637 NZ_CP033909:c2182153-2181746 [Mycobacterium avium subsp. paratuberculosis]
ATGACTGCAAGGCCGGCGGCCGGGGTGCTCGACACCTCGGTGTTCATCGCGTCCGAAAGCGGACGGCAGCTGGACGAGGC
GCGTATCCCCGACGAGGTAGCCACCACCGTCGTCACCCTTGCCGAACTTCACGCCGGCGTATTAGCCGCGACGACGTCCA
ACGTTCGGGCGCAACGCCTCGCAACGCTGGAATCGATAGCGGATATGGAAATGCTGCCAGTCGACGACGACGCGGTCCGC
ATGTGGGCAAGGTTGCGGATCCACCTAGCCGAGGCTGGACGGCGGATGCGGGCTAATGATCTGTGGATCGCGGCGATCGC
GGCATCGCGGGGACTGCCCGTTGTCACCCAGGATGACGACTTCGCCGCACTCGACGGCGCGGCGAACCTGGCGATTATTC
GGGTTTGA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9635.01 Da        Isoelectric Point: 11.7137

>AT114637 WP_003872399.1 NZ_CP033909:c2182410-2182150 [Mycobacterium avium subsp. paratuberculosis]
MSEVASRELRNDTAGVLRRVREGEDVTITINGRAVAMLTPLRPQRRRWLSKAELLSRLRRAQADPGLRQDLATLAGDTTE
DLGPIR

Download         Length: 261 bp

>AT114637 NZ_CP033909:c2182410-2182150 [Mycobacterium avium subsp. paratuberculosis]
ATGTCTGAAGTGGCGTCTCGCGAGCTGCGCAACGATACGGCGGGGGTACTGCGGCGCGTACGGGAAGGGGAGGACGTCAC
CATCACCATCAACGGCCGAGCCGTCGCGATGCTGACCCCGCTGCGGCCGCAGCGACGGCGGTGGCTGAGCAAGGCGGAGT
TGCTGTCGCGCTTGCGCCGCGCGCAAGCCGATCCCGGGCTACGCCAAGACCTCGCAACCCTTGCAGGCGACACCACTGAG
GACCTCGGTCCGATCCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L5MIC8

Antitoxin

Source ID Structure
AlphaFold DB A0A7L5MIC5

References