Detailed information of TA system TA114598

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4777022..4777548
• Replicon: chromosome
• Accession: NZ_CP033884
• Organism: Escherichia coli strain 50579417

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: EGM66_RS25680
• Protein ID: WP_000323025.1
• Coordinates: 4777022..4777309 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: EGM66_RS25685
• Protein ID: WP_000534858.1
• Coordinates: 4777309..4777548 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGM66_RS25635	4772046..4772261	-	216	WP_000839590.1	phage lysis protein EssD	-
EGM66_RS28330	4772481..4772651	+	171	WP_211180497.1	putative zinc-binding protein YnfU	-
EGM66_RS25640	4773015..4773230	-	216	WP_000066484.1	cold shock-like protein CspB	-
EGM66_RS25645	4773531..4773743	+	213	WP_000087756.1	cold shock-like protein CspF	-
EGM66_RS25650	4773798..4773887	+	90	WP_120795389.1	hypothetical protein	-
EGM66_RS25655	4774165..4774917	-	753	WP_001047135.1	antitermination protein	-
EGM66_RS25660	4774931..4775980	-	1050	WP_001265199.1	DUF968 domain-containing protein	-
EGM66_RS25665	4775982..4776260	-	279	WP_012304870.1	hypothetical protein	-
EGM66_RS25670	4776327..4776578	-	252	WP_000980994.1	hypothetical protein	-
EGM66_RS25675	4776795..4776950	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
EGM66_RS25680	4777022..4777309	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
EGM66_RS25685	4777309..4777548	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
EGM66_RS25690	4777573..4777878	+	306	WP_001326990.1	hypothetical protein	-
EGM66_RS25695	4778081..4778413	+	333	WP_001301033.1	protein FlxA	-
EGM66_RS25700	4778850..4780163	-	1314	WP_000589005.1	ISNCY family transposase	-
EGM66_RS25705	4780647..4781675	-	1029	WP_001345551.1	DUF4868 domain-containing protein	-
EGM66_RS25710	4781672..4782286	-	615	WP_001265627.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4744129..4803691	59562

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T114598 WP_000323025.1 NZ_CP033884:c4777309-4777022 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T114598 NZ_CP033884:c4777309-4777022 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT114598 WP_000534858.1 NZ_CP033884:c4777548-4777309 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT114598 NZ_CP033884:c4777548-4777309 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• AlphaFold DB: A0A4V1CTS8