Detailed information of TA system TA114597

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 4684171..4684809
• Replicon: chromosome
• Accession: NZ_CP033884
• Organism: Escherichia coli strain 50579417

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0D8WF76
• Locus tag: EGM66_RS25195
• Protein ID: WP_001306887.1
• Coordinates: 4684633..4684809 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: EGM66_RS25190
• Protein ID: WP_001270286.1
• Coordinates: 4684171..4684587 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGM66_RS25170	4679323..4680264	-	942	WP_001251315.1	ABC transporter permease	-
EGM66_RS25175	4680265..4681278	-	1014	WP_001523231.1	ABC transporter ATP-binding protein	-
EGM66_RS25180	4681296..4682441	-	1146	WP_020233879.1	ABC transporter substrate-binding protein	-
EGM66_RS25185	4682686..4684092	-	1407	WP_001523229.1	PLP-dependent aminotransferase family protein	-
EGM66_RS25190	4684171..4684587	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
EGM66_RS25195	4684633..4684809	-	177	WP_001306887.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
EGM66_RS25200	4685031..4685261	+	231	WP_000494241.1	YncJ family protein	-
EGM66_RS25205	4685353..4687314	-	1962	WP_057950830.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
EGM66_RS25210	4687387..4687923	-	537	WP_020233877.1	DNA-binding transcriptional regulator SutR	-
EGM66_RS25215	4687976..4689187	+	1212	WP_023143671.1	BenE family transporter YdcO	-
EGM66_RS25220	4689295..4689753	-	459	WP_000526135.1	IS200/IS605 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4689295..4689753	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6768.88 Da
• Isoelectric Point: 11.5336

>T114597 WP_001306887.1 NZ_CP033884:c4684809-4684633 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFRGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T114597 NZ_CP033884:c4684809-4684633 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCGTGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT114597 WP_001270286.1 NZ_CP033884:c4684587-4684171 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT114597 NZ_CP033884:c4684587-4684171 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0D8WF76