Detailed information of TA system TA114584

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 2984249..2984736
• Replicon: chromosome
• Accession: NZ_CP033884
• Organism: Escherichia coli strain 50579417

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: EGM66_RS16845
• Protein ID: WP_001520461.1
• Coordinates: 2984485..2984736 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A2T1MJK4
• Locus tag: EGM66_RS16840
• Protein ID: WP_001520458.1
• Coordinates: 2984249..2984482 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGM66_RS16815	2979322..2979609	+	288	WP_000203747.1	ferredoxin-like protein FixX	-
EGM66_RS16820	2979668..2980999	+	1332	WP_001520455.1	MFS transporter	-
EGM66_RS16825	2981107..2981637	+	531	WP_021522993.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
EGM66_RS16830	2981630..2983492	+	1863	WP_000377113.1	glutathione-regulated potassium-efflux system protein KefC	-
EGM66_RS16835	2983684..2984163	+	480	WP_000624375.1	type 3 dihydrofolate reductase	-
EGM66_RS16840	2984249..2984482	+	234	WP_001520458.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
EGM66_RS16845	2984485..2984736	+	252	WP_001520461.1	CcdB family protein	Toxin
EGM66_RS16850	2984796..2985644	-	849	WP_000257196.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
EGM66_RS16855	2985651..2986028	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
EGM66_RS16860	2986031..2986852	-	822	WP_001065381.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
EGM66_RS16865	2986849..2987838	-	990	WP_001520462.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
EGM66_RS16870	2987838..2989124	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-82)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9482.12 Da
• Isoelectric Point: 10.4134

>T114584 WP_001520461.1 NZ_CP033884:2984485-2984736 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKF

Nucleotide

• Length: 252 bp

>T114584 NZ_CP033884:2984485-2984736 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATTCTTTTACTGGTAGATGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8560.61 Da
• Isoelectric Point: 5.8062

>AT114584 WP_001520458.1 NZ_CP033884:2984249-2984482 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLMVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT114584 NZ_CP033884:2984249-2984482 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTATGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A2T1MJK4