Detailed information of TA system TA114583

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 2821080..2821894
• Replicon: chromosome
• Accession: NZ_CP033884
• Organism: Escherichia coli strain 50579417

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: EGM66_RS16060
• Protein ID: WP_001054376.1
• Coordinates: 2821637..2821894 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: EGM66_RS16055
• Protein ID: WP_001309181.1
• Coordinates: 2821080..2821625 (-)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGM66_RS16030	2816771..2818084	-	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-
EGM66_RS16035	2818096..2818374	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
EGM66_RS16040	2818371..2819492	-	1122	WP_000010829.1	M42 family metallopeptidase	-
EGM66_RS28140	2819737..2819853	-	117	Protein_2609	VOC family protein	-
EGM66_RS16045	2819891..2820109	-	219	Protein_2610	hypothetical protein	-
EGM66_RS16050	2820278..2821024	-	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
EGM66_RS16055	2821080..2821625	-	546	WP_001309181.1	N-acetyltransferase	Antitoxin
EGM66_RS16060	2821637..2821894	-	258	WP_001054376.1	hypothetical protein	Toxin
EGM66_RS16065	2822271..2822516	-	246	Protein_2614	GNAT family N-acetyltransferase	-
EGM66_RS16070	2822632..2823872	+	1241	Protein_2615	helicase YjhR	-
EGM66_RS16075	2824455..2825435	-	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
EGM66_RS16080	2825500..2826606	-	1107	WP_001309184.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T114583 WP_001054376.1 NZ_CP033884:c2821894-2821637 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T114583 NZ_CP033884:c2821894-2821637 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT114583 WP_001309181.1 NZ_CP033884:c2821625-2821080 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT114583 NZ_CP033884:c2821625-2821080 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8