Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1109565..1110219 Replicon chromosome
Accession NZ_CP033884
Organism Escherichia coli strain 50579417

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag EGM66_RS07640 Protein ID WP_000244781.1
Coordinates 1109565..1109972 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag EGM66_RS07645 Protein ID WP_000354046.1
Coordinates 1109953..1110219 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGM66_RS07620 1105522..1107255 - 1734 WP_000813233.1 single-stranded-DNA-specific exonuclease RecJ -
EGM66_RS07625 1107261..1107971 - 711 WP_000715222.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EGM66_RS07630 1107996..1108892 - 897 WP_000806650.1 site-specific tyrosine recombinase XerD -
EGM66_RS07635 1109004..1109525 + 522 WP_001055874.1 flavodoxin FldB -
EGM66_RS07640 1109565..1109972 - 408 WP_000244781.1 protein YgfX Toxin
EGM66_RS07645 1109953..1110219 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
EGM66_RS07650 1110462..1111442 + 981 WP_000886087.1 tRNA-modifying protein YgfZ -
EGM66_RS07655 1111519..1112178 - 660 WP_000250274.1 hemolysin III family protein -
EGM66_RS07660 1112342..1112653 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
EGM66_RS07665 1112698..1114131 + 1434 WP_001394742.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T114575 WP_000244781.1 NZ_CP033884:c1109972-1109565 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T114575 NZ_CP033884:c1109972-1109565 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGTG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGAGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT114575 WP_000354046.1 NZ_CP033884:c1110219-1109953 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT114575 NZ_CP033884:c1110219-1109953 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTCAACTGGCTGATGAATCATGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References