Detailed information of TA system TA114565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 30090..30637
• Replicon: plasmid p50579417_4
• Accession: NZ_CP033881
• Organism: Escherichia coli strain 50579417

Toxin (Protein)

• Gene name: parE
• Uniprot ID: E9LLH3
• Locus tag: EGM66_RS00920
• Protein ID: WP_000969996.1
• Coordinates: 30090..30371 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: EGM66_RS00925
• Protein ID: WP_001481137.1
• Coordinates: 30368..30637 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGM66_RS00890	25305..26207	-	903	WP_001386431.1	DUF1281 domain-containing protein	-
EGM66_RS00895	26744..27706	+	963	WP_000959884.1	plasmid segregation protein ParM	-
EGM66_RS00900	27709..28059	+	351	WP_001365718.1	plasmid partitioning/stability family protein	-
EGM66_RS00905	28172..28486	-	315	Protein_43	transposase	-
EGM66_RS00910	28608..29090	-	483	WP_001311056.1	hypothetical protein	-
EGM66_RS00915	29207..30055	-	849	WP_060578162.1	3'-5' exonuclease	-
EGM66_RS00920	30090..30371	-	282	WP_000969996.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EGM66_RS00925	30368..30637	-	270	WP_001481137.1	hypothetical protein	Antitoxin
EGM66_RS00940	31553..32410	-	858	WP_016240488.1	incFII family plasmid replication initiator RepA	-
EGM66_RS00945	32403..32477	-	75	WP_001365705.1	RepA leader peptide Tap	-
EGM66_RS00955	32722..32970	-	249	WP_000083839.1	replication regulatory protein RepA	-
EGM66_RS00960	33255..33404	-	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	-
-	33449..33508	+	60	NuclAT_1	-	-
-	33449..33508	+	60	NuclAT_1	-	-
-	33449..33508	+	60	NuclAT_1	-	-
-	33449..33508	+	60	NuclAT_1	-	-
EGM66_RS00970	33651..34124	-	474	WP_016240489.1	hypothetical protein	-
EGM66_RS00975	34279..34869	-	591	WP_033807967.1	DUF2726 domain-containing protein	-
EGM66_RS00980	34907..35116	-	210	WP_001298565.1	hemolysin expression modulator Hha	-
EGM66_RS00985	35162..35623	-	462	WP_001233838.1	thermonuclease family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..61890	61890

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10893.57 Da
• Isoelectric Point: 8.4615

>T114565 WP_000969996.1 NZ_CP033881:c30371-30090 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Nucleotide

• Length: 282 bp

>T114565 NZ_CP033881:c30371-30090 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10205.53 Da
• Isoelectric Point: 7.3210

>AT114565 WP_001481137.1 NZ_CP033881:c30637-30368 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLEHGDVLPHDMVESSATAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT114565 NZ_CP033881:c30637-30368 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCCCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCACGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCCGCTACTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: E9LLH3