Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 571..1163 Replicon plasmid p50579417_5
Accession NZ_CP033879
Organism Escherichia coli strain 50579417

Toxin (Protein)


Gene name hicA Uniprot ID V0AGQ2
Locus tag EGM66_RS00010 Protein ID WP_000520549.1
Coordinates 571..819 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID V0AWG0
Locus tag EGM66_RS00015 Protein ID WP_000681613.1
Coordinates 816..1163 (+) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGM66_RS00010 571..819 + 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin Toxin
EGM66_RS00015 816..1163 + 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
EGM66_RS00025 1526..2029 + 504 WP_000781817.1 hypothetical protein -
EGM66_RS00030 2033..3262 + 1230 WP_001329504.1 relaxase/mobilization nuclease domain-containing protein -
EGM66_RS00035 3339..3632 + 294 WP_060578246.1 LuxR family transcriptional regulator -
EGM66_RS00040 3629..3844 + 216 WP_000760375.1 EexN family lipoprotein -
EGM66_RS00045 4201..4821 + 621 WP_000157548.1 lytic transglycosylase domain-containing protein -
EGM66_RS00050 4889..5161 + 273 WP_001329503.1 TrbC/VirB2 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..31882 31882


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9422.08 Da        Isoelectric Point: 10.4800

>T114560 WP_000520549.1 NZ_CP033879:571-819 [Escherichia coli]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Download         Length: 249 bp

>T114560 NZ_CP033879:571-819 [Escherichia coli]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13113.84 Da        Isoelectric Point: 4.6723

>AT114560 WP_000681613.1 NZ_CP033879:816-1163 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Download         Length: 348 bp

>AT114560 NZ_CP033879:816-1163 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CIX3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CLC1

References