Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT-BrnA
Location 19618..20206 Replicon plasmid pAb-MCR4.3
Accession NZ_CP033872
Organism Acinetobacter baumannii strain MRSN15313

Toxin (Protein)


Gene name brnT Uniprot ID A0A3G6Z3M6
Locus tag EGM95_RS20870 Protein ID WP_000438826.1
Coordinates 19919..20206 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID A0A335NKQ9
Locus tag EGM95_RS20865 Protein ID WP_024434403.1
Coordinates 19618..19932 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGM95_RS20850 15509..15964 + 456 WP_000932329.1 hypothetical protein -
EGM95_RS20855 16313..16774 - 462 WP_000761345.1 DIP1984 family protein -
EGM95_RS20860 17079..19490 - 2412 WP_000932953.1 TonB-dependent receptor ZnuD2 -
EGM95_RS20865 19618..19932 - 315 WP_024434403.1 BrnA antitoxin family protein Antitoxin
EGM95_RS20870 19919..20206 - 288 WP_000438826.1 BrnT family toxin Toxin
EGM95_RS20875 20579..21097 + 519 WP_000447193.1 sel1 repeat family protein -
EGM95_RS21190 21291..21425 - 135 Protein_18 DNA-binding protein -
EGM95_RS20895 22754..22897 + 144 Protein_20 DNA-directed DNA polymerase -
EGM95_RS20900 23062..23349 - 288 WP_005134734.1 type II toxin-antitoxin system RelE/ParE family toxin -
EGM95_RS20905 23339..23587 - 249 WP_005134732.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
EGM95_RS20910 23590..24021 - 432 WP_123794408.1 hypothetical protein -
EGM95_RS20915 24091..24705 - 615 WP_123794409.1 recombinase family protein -
EGM95_RS20920 24846..25055 + 210 WP_034121058.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-4.3 - 1..35502 35502


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(41-96)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11303.72 Da        Isoelectric Point: 6.0889

>T114553 WP_000438826.1 NZ_CP033872:c20206-19919 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Download         Length: 288 bp

>T114553 NZ_CP033872:c20206-19919 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 12222.86 Da        Isoelectric Point: 7.2026

>AT114553 WP_024434403.1 NZ_CP033872:c19932-19618 [Acinetobacter baumannii]
MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTIPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Download         Length: 315 bp

>AT114553 NZ_CP033872:c19932-19618 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACACAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGATTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
AAAATCAATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G6Z3M6

Antitoxin

Source ID Structure
AlphaFold DB A0A335NKQ9

References