Detailed information of TA system TA114548

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 427011..427690
• Replicon: chromosome
• Accession: NZ_CP033866
• Organism: Acinetobacter sp. FDAARGOS_494

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S3TWT7
• Locus tag: EG365_RS02140
• Protein ID: WP_000838146.1
• Coordinates: 427508..427690 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: N9L2H6
• Locus tag: EG365_RS02135
• Protein ID: WP_000966688.1
• Coordinates: 427011..427415 (-)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EG365_RS02100	422304..422819	-	516	WP_001056868.1	Rha family transcriptional regulator	-
EG365_RS02105	423160..424095	-	936	WP_000254125.1	ORF6N domain-containing protein	-
EG365_RS02110	424245..424511	+	267	WP_000774879.1	hypothetical protein	-
EG365_RS02115	424554..425045	-	492	WP_000755583.1	DUF4468 domain-containing protein	-
EG365_RS02120	425131..425877	-	747	WP_000599537.1	hypothetical protein	-
EG365_RS02125	425939..426322	-	384	WP_000725052.1	hypothetical protein	-
EG365_RS02130	426387..426911	-	525	WP_000721574.1	DUF4468 domain-containing protein	-
EG365_RS02135	427011..427415	-	405	WP_000966688.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EG365_RS02140	427508..427690	-	183	WP_000838146.1	type II toxin-antitoxin system HicA family toxin	Toxin
EG365_RS02150	428016..428531	-	516	WP_062939314.1	hypothetical protein	-
EG365_RS02155	428601..429518	-	918	WP_000094278.1	hypothetical protein	-
EG365_RS02160	429571..430749	-	1179	WP_000002408.1	hypothetical protein	-
EG365_RS02165	430749..431102	-	354	WP_000064593.1	hypothetical protein	-
EG365_RS02170	431199..431720	-	522	WP_000749907.1	SH3 domain-containing protein	-
EG365_RS02175	431829..432047	-	219	WP_001277696.1	hypothetical protein	-
EG365_RS02180	432049..432447	-	399	WP_001251864.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	408001..458673	50672

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6695.84 Da
• Isoelectric Point: 10.5523

>T114548 WP_000838146.1 NZ_CP033866:c427690-427508 [Acinetobacter sp. FDAARGOS_494]
VKSLDLIKMIEADGWYEVRVSGSHHHFKHPTKKGLVTIPHPKKDLPNGTVKSILKQAGLN

Nucleotide

• Length: 183 bp

>T114548 NZ_CP033866:c427690-427508 [Acinetobacter sp. FDAARGOS_494]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGATGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
TAAACACCCAACCAAAAAGGGGTTAGTTACAATCCCACATCCTAAAAAGGATTTACCAAACGGAACTGTTAAAAGCATTT
TGAAACAAGCGGGTCTAAATTGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14647.69 Da
• Isoelectric Point: 4.4328

>AT114548 WP_000966688.1 NZ_CP033866:c427415-427011 [Acinetobacter sp. FDAARGOS_494]
MLYPIAIERGSDTEAFGVTVPDIPGCFSAGDTLEEAIENVKEAISGHLEILAEDGEEIPLASELVKFVDDPEYKGMIWAV
TEVDVSRYLGKPEKINVTLPSRLIRKIDENVGKGKRYTTRSAFLAAGAEKLLHA

Nucleotide

• Length: 405 bp

>AT114548 NZ_CP033866:c427415-427011 [Acinetobacter sp. FDAARGOS_494]
ATGTTATATCCAATTGCAATTGAACGAGGATCAGATACTGAGGCATTTGGTGTCACTGTTCCTGATATTCCAGGTTGTTT
TAGTGCTGGTGACACACTTGAAGAAGCTATTGAGAATGTTAAAGAAGCTATTTCAGGCCATTTAGAAATATTGGCTGAAG
ATGGTGAGGAAATCCCATTAGCTTCCGAACTAGTTAAATTTGTCGATGATCCTGAATATAAAGGAATGATCTGGGCGGTT
ACCGAAGTTGATGTTAGTCGTTATCTGGGTAAACCAGAAAAAATCAATGTTACTTTACCAAGCCGTTTGATTCGTAAAAT
TGATGAGAATGTAGGTAAAGGTAAGAGATATACTACTCGATCGGCTTTCTTGGCTGCTGGTGCTGAAAAACTTTTACATG
CATAG

Structures

Toxin

• AlphaFold DB: S3TWT7

Antitoxin

• AlphaFold DB: N9L2H6