Detailed information of TA system TA114527

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2422696..2423375
• Replicon: chromosome
• Accession: NZ_CP033862
• Organism: Acinetobacter sp. FDAARGOS_560

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S3TWT7
• Locus tag: EG364_RS11935
• Protein ID: WP_000838146.1
• Coordinates: 2423193..2423375 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: N9L2H6
• Locus tag: EG364_RS11930
• Protein ID: WP_000966688.1
• Coordinates: 2422696..2423100 (-)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EG364_RS11895	2417989..2418504	-	516	WP_001056868.1	Rha family transcriptional regulator	-
EG364_RS11900	2418845..2419780	-	936	WP_000254125.1	ORF6N domain-containing protein	-
EG364_RS11905	2419930..2420196	+	267	WP_000774879.1	hypothetical protein	-
EG364_RS11910	2420239..2420730	-	492	WP_000755583.1	DUF4468 domain-containing protein	-
EG364_RS11915	2420816..2421562	-	747	WP_000599537.1	hypothetical protein	-
EG364_RS11920	2421624..2422007	-	384	WP_000725052.1	hypothetical protein	-
EG364_RS11925	2422072..2422596	-	525	WP_000721574.1	DUF4468 domain-containing protein	-
EG364_RS11930	2422696..2423100	-	405	WP_000966688.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EG364_RS11935	2423193..2423375	-	183	WP_000838146.1	type II toxin-antitoxin system HicA family toxin	Toxin
EG364_RS11945	2423701..2424216	-	516	WP_024616020.1	hypothetical protein	-
EG364_RS11950	2424287..2425204	-	918	WP_000094253.1	hypothetical protein	-
EG364_RS11955	2425257..2426612	-	1356	WP_000002415.1	hypothetical protein	-
EG364_RS11960	2426612..2426965	-	354	WP_000064593.1	hypothetical protein	-
EG364_RS11965	2427061..2427582	-	522	WP_000749909.1	SH3 domain-containing protein	-
EG364_RS11970	2427691..2427909	-	219	WP_001277696.1	hypothetical protein	-
EG364_RS11975	2427911..2428354	-	444	WP_002016455.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2403783..2453432	49649

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6695.84 Da
• Isoelectric Point: 10.5523

>T114527 WP_000838146.1 NZ_CP033862:c2423375-2423193 [Acinetobacter sp. FDAARGOS_560]
VKSLDLIKMIEADGWYEVRVSGSHHHFKHPTKKGLVTIPHPKKDLPNGTVKSILKQAGLN

Nucleotide

• Length: 183 bp

>T114527 NZ_CP033862:c2423375-2423193 [Acinetobacter sp. FDAARGOS_560]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGATGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
TAAACACCCAACCAAAAAGGGGTTAGTTACAATCCCACATCCTAAAAAGGATTTACCAAACGGAACTGTTAAAAGCATTT
TGAAACAAGCGGGTCTAAATTGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14647.69 Da
• Isoelectric Point: 4.4328

>AT114527 WP_000966688.1 NZ_CP033862:c2423100-2422696 [Acinetobacter sp. FDAARGOS_560]
MLYPIAIERGSDTEAFGVTVPDIPGCFSAGDTLEEAIENVKEAISGHLEILAEDGEEIPLASELVKFVDDPEYKGMIWAV
TEVDVSRYLGKPEKINVTLPSRLIRKIDENVGKGKRYTTRSAFLAAGAEKLLHA

Nucleotide

• Length: 405 bp

>AT114527 NZ_CP033862:c2423100-2422696 [Acinetobacter sp. FDAARGOS_560]
ATGTTATATCCAATTGCAATTGAACGAGGATCAGATACTGAGGCATTTGGTGTCACTGTTCCTGATATTCCAGGTTGTTT
TAGTGCTGGTGACACACTTGAAGAAGCTATTGAGAATGTTAAAGAAGCTATTTCAGGCCATTTAGAAATATTGGCTGAAG
ATGGTGAGGAAATCCCATTAGCTTCCGAACTAGTTAAATTTGTCGATGATCCTGAATATAAAGGAATGATCTGGGCGGTT
ACCGAAGTTGATGTTAGTCGTTATCTGGGTAAACCAGAAAAAATCAATGTTACTTTACCAAGCCGTTTGATTCGTAAAAT
TGATGAGAATGTAGGTAAAGGTAAGAGATATACTACTCGATCGGCTTTCTTGGCTGCTGGTGCTGAAAAACTTTTACATG
CATAG

Structures

Toxin

• AlphaFold DB: S3TWT7

Antitoxin

• AlphaFold DB: N9L2H6