Detailed information of TA system TA114526

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-PumB/upstrm_HI1419-dnstrm_HI1420
• Location: 3954377..3954985
• Replicon: chromosome
• Accession: NZ_CP033861
• Organism: Alcaligenes faecalis strain FDAARGOS_491

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A3G6HLB3
• Locus tag: EGY22_RS18275
• Protein ID: WP_042487248.1
• Coordinates: 3954377..3954667 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: EGY22_RS18280
• Protein ID: WP_042487247.1
• Coordinates: 3954680..3954985 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGY22_RS18260	3950316..3951791	-	1476	WP_042487254.1	NAD-dependent succinate-semialdehyde dehydrogenase	-
EGY22_RS18265	3952108..3952881	+	774	WP_042487251.1	IclR family transcriptional regulator	-
EGY22_RS18270	3952939..3954327	+	1389	WP_165589484.1	ATP-dependent RNA helicase DbpA	-
EGY22_RS18275	3954377..3954667	+	291	WP_042487248.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EGY22_RS18280	3954680..3954985	+	306	WP_042487247.1	putative addiction module antidote protein	Antitoxin
EGY22_RS18285	3955077..3956030	-	954	WP_042487243.1	LysR family transcriptional regulator	-
EGY22_RS18290	3956417..3957775	+	1359	WP_042487239.1	aromatic ring-hydroxylating dioxygenase subunit alpha	-
EGY22_RS18295	3957772..3958320	+	549	WP_042487236.1	3-phenylpropionate/cinnamic acid dioxygenase subunit beta	-
EGY22_RS18300	3958349..3958669	+	321	WP_026484793.1	bifunctional 3-phenylpropionate/cinnamic acid dioxygenase ferredoxin subunit	-
EGY22_RS18305	3958666..3959481	+	816	WP_042487233.1	3-(cis-5,6-dihydroxycyclohexa-1, 3-dien-1-yl)propanoate dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-95)

Antitoxin

• Uncharacterized protein HI1420 (5-93)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11029.53 Da
• Isoelectric Point: 6.7185

>T114526 WP_042487248.1 NZ_CP033861:3954377-3954667 [Alcaligenes faecalis]
MKTIHTIDTFDHWFGGLRDKQAARRIQVRIDRAEDGNFGDCEPVGEGVPEMRIHYGPGYRVYFVQRELEIVILLADGNKS
TQNKDIKTALEIARQL

Nucleotide

• Length: 291 bp

>T114526 NZ_CP033861:3954377-3954667 [Alcaligenes faecalis]
ATGAAGACTATCCACACCATCGATACCTTCGATCACTGGTTTGGCGGTCTGCGCGACAAGCAGGCCGCTAGACGGATTCA
GGTTCGTATTGACCGAGCTGAAGATGGCAACTTTGGAGACTGCGAGCCTGTCGGCGAAGGAGTCCCCGAAATGCGTATTC
ACTACGGACCTGGCTACCGTGTGTACTTTGTACAACGTGAACTGGAGATCGTGATCTTATTGGCGGATGGCAATAAATCT
ACCCAGAATAAAGATATTAAAACTGCGCTGGAAATCGCGCGGCAACTATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10940.59 Da
• Isoelectric Point: 5.6998

>AT114526 WP_042487247.1 NZ_CP033861:3954680-3954985 [Alcaligenes faecalis]
MGTIKLRKWDSAEYLQTEEDMVLYLEACMEEAGDDAAFIAKALGNIARAKGMSQLAKETGLGRESLYKALSGEGNPSFGT
ILKVMHALGIKLQPQLAPHNS

Nucleotide

• Length: 306 bp

>AT114526 NZ_CP033861:3954680-3954985 [Alcaligenes faecalis]
ATGGGAACCATCAAATTACGCAAATGGGATAGTGCCGAATACCTCCAAACAGAGGAAGACATGGTGCTCTACCTGGAAGC
CTGCATGGAAGAAGCGGGCGACGATGCCGCTTTCATTGCAAAAGCATTAGGAAATATTGCTCGCGCTAAAGGCATGAGCC
AACTGGCCAAAGAAACAGGCTTGGGACGTGAAAGCCTGTACAAAGCCCTGTCTGGCGAAGGCAATCCAAGCTTTGGCACC
ATTCTGAAAGTCATGCACGCTTTGGGAATCAAACTGCAACCGCAGCTTGCTCCCCACAACTCCTAA

Structures

Toxin

• AlphaFold DB: A0A3G6HLB3