Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 3959732..3960370 Replicon chromosome
Accession NZ_CP033850
Organism Escherichia coli strain FDAARGOS_497

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag EGY17_RS21135 Protein ID WP_000813794.1
Coordinates 3959732..3959908 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag EGY17_RS21140 Protein ID WP_001270286.1
Coordinates 3959954..3960370 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGY17_RS21115 3955354..3956565 - 1212 WP_071788120.1 BenE family transporter YdcO -
EGY17_RS21120 3956618..3957154 + 537 WP_000429148.1 DNA-binding transcriptional regulator SutR -
EGY17_RS21125 3957227..3959188 + 1962 WP_023909195.1 23S rRNA 5-hydroxycytidine C2501 synthase -
EGY17_RS21130 3959280..3959510 - 231 WP_000491567.1 DUF2554 family protein -
EGY17_RS21135 3959732..3959908 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
EGY17_RS21140 3959954..3960370 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
EGY17_RS21145 3960449..3961855 + 1407 WP_022645665.1 PLP-dependent aminotransferase family protein -
EGY17_RS21150 3962100..3963245 + 1146 WP_000047466.1 ABC transporter substrate-binding protein -
EGY17_RS21155 3963263..3964276 + 1014 WP_022645666.1 ABC transporter ATP-binding protein -
EGY17_RS21160 3964277..3965218 + 942 WP_022645667.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T114514 WP_000813794.1 NZ_CP033850:3959732-3959908 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T114514 NZ_CP033850:3959732-3959908 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTGAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT114514 WP_001270286.1 NZ_CP033850:3959954-3960370 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT114514 NZ_CP033850:3959954-3960370 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAACAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CACTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References