114504

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2828655..2829273 Replicon chromosome
Accession NZ_CP033850
Organism Escherichia coli strain FDAARGOS_497

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag EGY17_RS15510 Protein ID WP_001291435.1
Coordinates 2828655..2828873 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag EGY17_RS15515 Protein ID WP_000344800.1
Coordinates 2828899..2829273 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGY17_RS15475 2823944..2824516 + 573 WP_000779831.1 YbaY family lipoprotein -
EGY17_RS15480 2824547..2824858 - 312 WP_000409911.1 MGMT family protein -
EGY17_RS15490 2825237..2825590 + 354 WP_000878140.1 DUF1428 domain-containing protein -
EGY17_RS15495 2825632..2827182 - 1551 WP_022645279.1 cyclic-guanylate-specific phosphodiesterase PdeB -
EGY17_RS15500 2827346..2827816 - 471 WP_022645280.1 YlaC family protein -
EGY17_RS15505 2827932..2828483 - 552 WP_000102574.1 maltose O-acetyltransferase -
EGY17_RS15510 2828655..2828873 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
EGY17_RS15515 2828899..2829273 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
EGY17_RS15520 2829819..2832968 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
EGY17_RS15525 2832991..2834184 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T114504 WP_001291435.1 NZ_CP033850:c2828873-2828655 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T114504 NZ_CP033850:c2828873-2828655 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACTATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT114504 WP_000344800.1 NZ_CP033850:c2829273-2828899 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT114504 NZ_CP033850:c2829273-2828899 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References