Detailed information of TA system TA114497

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 2219731..2220545
• Replicon: chromosome
• Accession: NZ_CP033850
• Organism: Escherichia coli strain FDAARGOS_497

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: EGY17_RS12680
• Protein ID: WP_001054376.1
• Coordinates: 2220288..2220545 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: EGY17_RS12675
• Protein ID: WP_001309181.1
• Coordinates: 2219731..2220276 (-)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGY17_RS12650	2215422..2216735	-	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-
EGY17_RS12655	2216747..2217025	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
EGY17_RS12660	2217022..2218143	-	1122	WP_000010829.1	M42 family metallopeptidase	-
EGY17_RS27555	2218388..2218504	-	117	Protein_2070	VOC family protein	-
EGY17_RS12665	2218542..2218760	-	219	Protein_2071	hypothetical protein	-
EGY17_RS12670	2218929..2219675	-	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
EGY17_RS12675	2219731..2220276	-	546	WP_001309181.1	N-acetyltransferase	Antitoxin
EGY17_RS12680	2220288..2220545	-	258	WP_001054376.1	hypothetical protein	Toxin
EGY17_RS12685	2220922..2221167	-	246	Protein_2075	GNAT family N-acetyltransferase	-
EGY17_RS12690	2221283..2222523	+	1241	Protein_2076	helicase YjhR	-
EGY17_RS12695	2222710..2223690	+	981	WP_000019400.1	IS5-like element IS5 family transposase	-
EGY17_RS12700	2224305..2225285	-	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T114497 WP_001054376.1 NZ_CP033850:c2220545-2220288 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T114497 NZ_CP033850:c2220545-2220288 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT114497 WP_001309181.1 NZ_CP033850:c2220276-2219731 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT114497 NZ_CP033850:c2220276-2219731 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8