Detailed information of TA system TA114496

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 2106423..2107018
• Replicon: chromosome
• Accession: NZ_CP033850
• Organism: Escherichia coli strain FDAARGOS_497

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: EGY17_RS12050
• Protein ID: WP_000239581.1
• Coordinates: 2106668..2107018 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: EGY17_RS12045
• Protein ID: WP_001223213.1
• Coordinates: 2106423..2106674 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGY17_RS12035	2102088..2105867	+	3780	WP_022646473.1	autotransporter assembly complex protein TamB	-
EGY17_RS12040	2105870..2106211	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
EGY17_RS12045	2106423..2106674	+	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
EGY17_RS12050	2106668..2107018	+	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
EGY17_RS12055	2107097..2107627	-	531	WP_000055075.1	inorganic diphosphatase	-
EGY17_RS12060	2107937..2108893	+	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
EGY17_RS12065	2109026..2110528	+	1503	WP_022646474.1	sugar ABC transporter ATP-binding protein	-
EGY17_RS12070	2110542..2111564	+	1023	WP_001298067.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T114496 WP_000239581.1 NZ_CP033850:2106668-2107018 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T114496 NZ_CP033850:2106668-2107018 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT114496 WP_001223213.1 NZ_CP033850:2106423-2106674 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT114496 NZ_CP033850:2106423-2106674 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7