Detailed information of TA system TA114485

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 40480..41081
• Replicon: plasmid unnamed2
• Accession: NZ_CP033848
• Organism: Escherichia coli strain FDAARGOS_497

Toxin (Protein)

• Gene name: doc
• Uniprot ID: U9Z1Q0
• Locus tag: EGY17_RS01150
• Protein ID: WP_001216047.1
• Coordinates: 40480..40860 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: EGY17_RS01155
• Protein ID: WP_001190712.1
• Coordinates: 40860..41081 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGY17_RS01125	35921..37405	-	1485	WP_000124159.1	hypothetical protein	-
EGY17_RS01130	37405..38598	-	1194	WP_000219618.1	hypothetical protein	-
EGY17_RS01135	38684..39136	-	453	WP_001326849.1	late promoter-activating protein	-
EGY17_RS01140	39225..40268	-	1044	WP_000648833.1	DUF968 domain-containing protein	-
EGY17_RS01145	40296..40475	-	180	WP_000113018.1	hypothetical protein	-
EGY17_RS01150	40480..40860	-	381	WP_001216047.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
EGY17_RS01155	40860..41081	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
EGY17_RS01160	41154..41543	-	390	WP_000506730.1	DNA repair protein	-
EGY17_RS01165	41667..41918	-	252	WP_001283837.1	DNA polymerase III subunit theta	-
EGY17_RS01170	42092..42301	+	210	WP_001344848.1	hypothetical protein	-
EGY17_RS01175	42286..42570	-	285	WP_123907670.1	antitoxin PHD	-
EGY17_RS01180	42554..43492	-	939	WP_123907671.1	hypothetical protein	-
EGY17_RS01185	43474..43848	-	375	WP_123907672.1	hypothetical protein	-
EGY17_RS01190	43855..44148	-	294	WP_073516126.1	hypothetical protein	-
EGY17_RS01195	44327..44560	-	234	WP_000517421.1	hypothetical protein	-
EGY17_RS01205	44646..44906	-	261	WP_033553819.1	hypothetical protein	-
EGY17_RS01210	44903..45784	-	882	WP_123907673.1	DUF551 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..97018	97018

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13574.27 Da
• Isoelectric Point: 5.1514

>T114485 WP_001216047.1 NZ_CP033848:c40860-40480 [Escherichia coli]
MRHISPEELVALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T114485 NZ_CP033848:c40860-40480 [Escherichia coli]
ATGAGACATATATCACCGGAAGAACTTGTTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCCGCAGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT114485 WP_001190712.1 NZ_CP033848:c41081-40860 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT114485 NZ_CP033848:c41081-40860 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: U9Z1Q0

Antitoxin

• AlphaFold DB: A0A829CJB6